Shufang Wei1, Weiyuan Ma2. 1. Department of Dermatology, Qilu Hospital of Shandong University, China. 2. Department of Dermatology, Qilu Hospital of Shandong University, China. Electronic address: wymsdql@163.com.
Abstract
BACKGROUND: Accumulating evidence has shown that miR-370 play an important role in the development and progression of tumor. However, the role of miR-370 in melanoma remains largely unknown. The present study is designed to investigate the function of miR-370 in melanoma and to explore the molecular mechanism underlying its function. MATERIALS AND METHODS: The expression level of miR-370 was detected in melanoma tissues and cell lines by real-time quantitative polymerase chain reaction (qRT-PCR). The effect of overexpression of miR-370 on in vitro cell proliferation, apoptosis, invasion as well as glyclolysis was examined. Western blotting analysis was used to detect the influence of miR-370 on the expression of target genes, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-370 in melanoma tissues. RESULTS: Our study showed that miR-370 was upregulated in melanoma tissues compared with non-cancerous tissues (P<0.01). In addition, the expression of miR-370 in melanoma cell lines was also significantly higher (P<0.01). Enforced expression of miR-370 promotes melanoma cell proliferation, inhibits apoptosis and enhances invasion and glycolysis and led to downregulation of the PDHB protein. Moreover, the expression level of miR-370 in melanoma tissues showed inverse relationship with the expression level of PDHB protein. CONCLUSIONS: In conclusion, our findings suggested that miR-370 represents a potential oncogenic miRNA and plays an important role in melanoma progression by directly targeting PDHB.
BACKGROUND: Accumulating evidence has shown that miR-370 play an important role in the development and progression of tumor. However, the role of miR-370 in melanoma remains largely unknown. The present study is designed to investigate the function of miR-370 in melanoma and to explore the molecular mechanism underlying its function. MATERIALS AND METHODS: The expression level of miR-370 was detected in melanoma tissues and cell lines by real-time quantitative polymerase chain reaction (qRT-PCR). The effect of overexpression of miR-370 on in vitro cell proliferation, apoptosis, invasion as well as glyclolysis was examined. Western blotting analysis was used to detect the influence of miR-370 on the expression of target genes, and Pearson analysis was used to calculate the correlation between the expression of targets gene and miR-370 in melanoma tissues. RESULTS: Our study showed that miR-370 was upregulated in melanoma tissues compared with non-cancerous tissues (P<0.01). In addition, the expression of miR-370 in melanoma cell lines was also significantly higher (P<0.01). Enforced expression of miR-370 promotes melanoma cell proliferation, inhibits apoptosis and enhances invasion and glycolysis and led to downregulation of the PDHB protein. Moreover, the expression level of miR-370 in melanoma tissues showed inverse relationship with the expression level of PDHB protein. CONCLUSIONS: In conclusion, our findings suggested that miR-370 represents a potential oncogenic miRNA and plays an important role in melanoma progression by directly targeting PDHB.
Authors: Daniël P de Bruyn; Aaron B Beasley; Robert M Verdijk; Natasha M van Poppelen; Dion Paridaens; Ronald O B de Keizer; Nicole C Naus; Elin S Gray; Annelies de Klein; Erwin Brosens; Emine Kiliç Journal: Biomedicines Date: 2022-02-21