Literature DB >> 28364250

Ultrasensitive Hybridization-Based ELISA Method for the Determination of Phosphorodiamidate Morpholino Oligonucleotides in Biological samples.

Umar Burki1, Volker Straub2.   

Abstract

Determining the concentration of oligonucleotide in biological samples such as tissue lysate and serum is essential for determining the biodistribution and pharmacokinetic profile, respectively. ELISA-based assays have shown far greater sensitivities compared to other methods such as HPLC and LC/MS. Here, we describe a novel ultrasensitive hybridization-based ELISA method for quantitating morpholino oligonucleotides in mouse tissue lysate and serum samples. The assay has a linear detection range of 5-250 pM (R2 > 0.99).

Entities:  

Keywords:  Biodistribution; ELISA; Hybridization; MNase; Pharmacokinetic; Phosphorodiamidate morpholino oligonucleotides

Mesh：

Substances：

Year:  2017        PMID： 28364250     DOI： 10.1007/978-1-4939-6817-6_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

Review 1.  A critical analysis of methods used to investigate the cellular uptake and subcellular localization of RNA therapeutics.

Authors:  Kirsten Deprey; Nefeli Batistatou; Joshua A Kritzer
Journal:  Nucleic Acids Res       Date:  2020-08-20       Impact factor: 16.971

2.  Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.

Authors:  Alison M Blain; Elizabeth Greally; Graham McClorey; Raquel Manzano; Corinne A Betts; Caroline Godfrey; Liz O'Donovan; Thibault Coursindel; Mike J Gait; Matthew J Wood; Guy A MacGowan; Volker W Straub
Journal:  PLoS One       Date:  2018-06-18       Impact factor: 3.240

3.  Proof of concept of peptide-linked blockmiR-induced MBNL functional rescue in myotonic dystrophy type 1 mouse model.

Authors:  Sarah J Overby; Estefanía Cerro-Herreros; Irene González-Martínez; Miguel A Varela; David Seoane-Miraz; Yahya Jad; Richard Raz; Thorleif Møller; Manuel Pérez-Alonso; Matthew J Wood; Beatriz Llamusí; Rubén Artero
Journal:  Mol Ther Nucleic Acids       Date:  2022-02-10       Impact factor: 8.886
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.