| Literature DB >> 28364241 |
Madzia P Crossley1,2, Torsten Krude3.
Abstract
Noncoding RNAs have essential biochemical functions in different areas of cellular metabolism, including protein synthesis, RNA splicing, protein secretion, and DNA replication. We have successfully used Morpholino antisense oligonucleotides for the functional inactivation of small noncoding RNAs required for DNA replication (Y RNAs in vertebrates and stem-bulge RNAs in nematodes). Here we discuss specific issues of targeting functional noncoding RNAs for inactivation by Morpholino antisense oligonucleotides. We present protocols for the design, preparation, and efficacy controls of Morpholino antisense oligonucleotides, as well as brief descriptions for their delivery into vertebrate and nematode embryos.Entities:
Keywords: Caenorhabditis elegans; Danio rerio; Dominant-negative inhibition; Functional assay to test Morpholino efficacy and specificity; Functional inhibition; Morpholino delivery; Morpholino design; Noncoding RNA domains; Noncoding RNAs; Xenopus laevis
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Year: 2017 PMID： 28364241 DOI： 10.1007/978-1-4939-6817-6_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745