Literature DB >> 28364238

Inhibition of Bacterial Growth by Peptide-Conjugated Morpholino Oligomers.

Seth M Daly1, Carolyn R Sturge1, David E Greenberg2,3.   

Abstract

Morpholino oligomers (MOs) are antisense molecules designed for sequence-specific binding of target mRNA. In bacteria, inhibition is hypothesized to occur by preventing translation initiation. Cell-penetrating peptides may be conjugated to the 5'- or 3'-termini of an MO to enhance cellular entry and therefore inhibition. Here we describe the three standard microbiological assays to assess in vitro antibacterial MO efficacy.

Entities:  

Keywords:  Antisense; Checkerboard synergy; Minimum bactericidal concentration (MBC); Minimum inhibitory concentration (MIC); Morpholino oligomers (MOs)

Mesh：

Substances：

Year:  2017        PMID： 28364238      PMCID： PMC5497461          DOI： 10.1007/978-1-4939-6817-6_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

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Authors:  G B ELION; S SINGER; G H HITCHINGS
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Authors:  David E Greenberg; Kimberly R Marshall-Batty; Lauren R Brinster; Kol A Zarember; Pamela A Shaw; Brett L Mellbye; Patrick L Iversen; Steven M Holland; Bruce L Geller
Journal:  J Infect Dis       Date:  2010-06-15       Impact factor: 5.226

3.  Inhibition of Pseudomonas aeruginosa by Peptide-Conjugated Phosphorodiamidate Morpholino Oligomers.

Authors:  James J Howard; Carolyn R Sturge; Dina A Moustafa; Seth M Daly; Kimberly R Marshall-Batty; Christina F Felder; Danniel Zamora; Marium Yabe-Gill; Maria Labandeira-Rey; Stacey M Bailey; Michael Wong; Joanna B Goldberg; Bruce L Geller; David E Greenberg
Journal:  Antimicrob Agents Chemother       Date:  2017-03-24       Impact factor: 5.191

4.  Bactericidal antisense effects of peptide-PNA conjugates.

Authors:  L Good; S K Awasthi; R Dryselius; O Larsson; P E Nielsen
Journal:  Nat Biotechnol       Date:  2001-04       Impact factor: 54.908

Review 5.  Rationale for use of antimicrobial combinations.

Authors:  R C Moellering
Journal:  Am J Med       Date:  1983-08-29       Impact factor: 4.965

6.  Gene-silencing antisense oligomers inhibit acinetobacter growth in vitro and in vivo.

Authors:  Bruce L Geller; Kimberly Marshall-Batty; Frederick J Schnell; Mattie M McKnight; Patrick L Iversen; David E Greenberg
Journal:  J Infect Dis       Date:  2013-10-14       Impact factor: 5.226

7.  Cell-penetrating peptides as transporters for morpholino oligomers: effects of amino acid composition on intracellular delivery and cytotoxicity.

Authors:  Rebecca P Wu; Derek S Youngblood; Jed N Hassinger; Candace E Lovejoy; Michelle H Nelson; Patrick L Iversen; Hong M Moulton
Journal:  Nucleic Acids Res       Date:  2007-08-01       Impact factor: 16.971
  7 in total
  6 in total

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Review 3.  Antisense Phosphorodiamidate Morpholino Oligomers as Novel Antiviral Compounds.

Authors:  Yuchen Nan; Yan-Jin Zhang
Journal:  Front Microbiol       Date:  2018-04-20       Impact factor: 5.640

4.  A New Antisense Phosphoryl Guanidine Oligo-2'-O-Methylribonucleotide Penetrates Into Intracellular Mycobacteria and Suppresses Target Gene Expression.

Authors:  Yulia V Skvortsova; Elena G Salina; Ekaterina A Burakova; Oksana S Bychenko; Dmitry A Stetsenko; Tatyana L Azhikina
Journal:  Front Pharmacol       Date:  2019-09-19       Impact factor: 5.810

5.  Extraction, Purification, and Characterization of Polysaccharides of Araucaria heterophylla L and Prosopis chilensis L and Utilization of Polysaccharides in Nanocarrier Synthesis.

Authors:  Antony V Samrot; Teeshalini Kudaiyappan; Ummu Bisyarah; Anita Mirarmandi; Etel Faradjeva; Amira Abubakar; Jenifer A Selvarani; Suresh Kumar Subbiah
Journal:  Int J Nanomedicine       Date:  2020-09-25

6.  Multi-drug resistant bacteria isolates from lymphatic filariasis patients in the Ahanta West District, Ghana.

Authors:  Bill Clinton Aglomasa; Cynthia Kyerewaa Adu-Asiamah; Samuel Opoku Asiedu; Priscilla Kini; Emmanuel Kobla Atsu Amewu; Kennedy Gyau Boahen; Solomon Wireko; Isaac Kingsley Amponsah; Yaw Duah Boakye; Vivian Etsiapa Boamah; Alexander Kwarteng
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  6 in total

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