Literature DB >> 28361431

Modification of Rat Lung Decellularization Protocol Based on Dynamic Conductometry of Working Solution.

E V Kuevda1, E A Gubareva2, I S Gumenyuk2, A S Sotnichenko2, I V Gilevich2, R Z Nakokhov2, T V Rusinova2, T G Yudina2, A N Red'ko2, S N Alekseenko2.   

Abstract

We modified the protocol of obtaining of biological scaffolds of rat lungs based on dynamic recording of specific resistivity of working detergent solution (conductometry) during perfusion decellularization. Termination of sodium deoxycholate exposure after attaining ionic equilibrium plateau did not impair the quality of decellularization and preserved structural matrix components, which was confirmed by morphological analysis and quantitative assay of residual DNA.

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Keywords:  biological carcasses; conductometry; lung decellularization; rats; sodium deoxycholate

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Year:  2017        PMID： 28361431     DOI： 10.1007/s10517-017-3692-3

Source DB:  PubMed          Journal:  Bull Exp Biol Med        ISSN： 0007-4888            Impact factor:   0.804


  1 in total

1.  Fabrication and Characterization of Alveolus-Like Scaffolds with Control of the Pore Architecture and Gas Permeability.

Authors:  Lanxin Lü; Hongxian Shen; Daichi Kasai; Ying Yang
Journal:  Stem Cells Int       Date:  2022-01-20       Impact factor: 5.443
  1 in total

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