Reza Fekrazad1, Bahman Seraj2, Nasim Chiniforush3, Mehrak Rokouei4, Niloofar Mousavi3, Sara Ghadimi5. 1. Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Islamic Republic of Iran. 2. Dental Research Center Dental Research Institute, Department of Pediatric dentistry Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran. 3. Laser Research Center of Dentistry Dental Research Institute, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran. 4. Department of Pediatric Dentistry School of Dentistry, Golestan University of Medical Sciences, Gorgan, Islamic Republic of Iran. 5. Laser Research Center of Dentistry, Dental Research Institute,Department of Pediatric Dentistry, School of Dentistry, Tehran University of Medical Sciences, North Amirabad St.,Tehran, Islamic Republic of Iran. Electronic address: sghadimi@tums.ac.ir.
Abstract
BACKGROUND: Antimicrobial photodynamic therapy (aPDT) is a novel technique for reduction of pathogenic microorganisms in dentistry. The aim of this study was to evaluate the effects of aPDT on Streptococcus mutans reduction in children with severe early childhood caries. METHODS: Twenty-two children with severe early childhood caries aged 3-6 years were treated with toluoidine blue O (TBO) for 1min and irradiated by a Light Emitting Diode (LED; FotoSan, CMS Dental, Denmark) with the exposure time of 150s. Saliva samples were collected at baseline, 1h and 7 days after treatment. S. mutans counts were determined using the Dentocult SM Strip mutans. RESULTS: The counts of S. mutans in saliva decreased significantly after 1h (P<0.001). However, the difference in reduction of S. mutans counts in saliva was not significant between the baseline and 7 days after treatment (P>0.05). CONCLUSION: aPDT seems to be efficient to reduce salivary S. mutans immediately after treatment in children with severe early childhood caries. However, further research is needed to evaluate different doses and frequency of irradiation in combination with restoring carious teeth to find more durable results.
BACKGROUND: Antimicrobial photodynamic therapy (aPDT) is a novel technique for reduction of pathogenic microorganisms in dentistry. The aim of this study was to evaluate the effects of aPDT on Streptococcus mutans reduction in children with severe early childhood caries. METHODS: Twenty-two children with severe early childhood caries aged 3-6 years were treated with toluoidine blue O (TBO) for 1min and irradiated by a Light Emitting Diode (LED; FotoSan, CMS Dental, Denmark) with the exposure time of 150s. Saliva samples were collected at baseline, 1h and 7 days after treatment. S. mutans counts were determined using the Dentocult SM Strip mutans. RESULTS: The counts of S. mutans in saliva decreased significantly after 1h (P<0.001). However, the difference in reduction of S. mutans counts in saliva was not significant between the baseline and 7 days after treatment (P>0.05). CONCLUSION: aPDT seems to be efficient to reduce salivary S. mutans immediately after treatment in children with severe early childhood caries. However, further research is needed to evaluate different doses and frequency of irradiation in combination with restoring carious teeth to find more durable results.
Authors: Abdulrahman A Balhaddad; Mohammed S AlQranei; Maria S Ibrahim; Michael D Weir; Frederico C Martinho; Hockin H K Xu; Mary Anne S Melo Journal: Int J Mol Sci Date: 2020-10-15 Impact factor: 5.923