| Literature DB >> 28359875 |
M S Regueira1, Saulo Relison Tintino2, Ana Raquel Pereira da Silva2, Maria do Socorro Costa2, Aline Augusti Boligon3, Edinardo F F Matias4, Valdir de Queiroz Balbino5, Irwin R A Menezes2, Henrique Douglas Melo Coutinho2.
Abstract
The aim of this study was to investigate the effect of the dry and rainy season on the antibacterial activity and chemical composition of the Brazilian red propolis. The samples were collected in rainy (RP-PER) and dry (RP-PED) seasons and analyzed by HPLC-DAD. The extracts were tested alone and in association with antibiotics against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The HPLC analysis identified luteolin and quercetin as the main compounds. Seasonal variation was observed according to concentrations of the compounds. The MIC values against E. coli ranged from 128 μg/mL to 512 μg/mL (EC 06 and EC ATCC). The red propolis showed MIC values of 512 μg/mL against both strains of P. aeruginosa used in our study (PA03 and PA24) and against strains of Gram-positive bacteria S. aureus the MICs ranged from 64 μg/mL to ≥1024 μg/mL (SA10). A synergistic effect was observed when we combined the RP-PED with gentamicin against all the strains tested. When we combined the RP-PED with Imipenem, we only observed synergistic effect against P. aeruginosa. According to our synergistic activity results, the utilization of red propolis collected in the drier periods can be used as an adjuvant against multiresistant bacterial infections.Entities:
Keywords: Antibacterial activity; Phenolic compounds; Red propolis; Seasonal variation; Synergistic effect
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Year: 2017 PMID： 28359875 DOI： 10.1016/j.fct.2017.03.052
Source DB: PubMed Journal: Food Chem Toxicol ISSN： 0278-6915 Impact factor: 6.023