Milda Naciute1, Gabriele Maciunaite1, Diana Mieliauskaite1, Rita Rugiene1,2, Aukse Zinkeviciene1, Mykolas Mauricas1, Modra Murovska3, Irute Girkontaite4. 1. Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania. 2. Centre of Rheumatology, Vilnius University, Vilnius, Lithuania. 3. A. Kirchenstein Institute of Microbiology and Virology, Riga Stradins University, Riga, Latvia. 4. Department of Immunology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania igirkontaite@yahoo.com.
Abstract
AIM: To investigate T-cell subpopulations in peripheral blood of human parvovirus B19 DNA-positive (B19+) and -negative (B19-) patients with rheumatoid arthritis (RA) and healthy persons. PATIENTS AND METHODS: Blood samples were collected from 115 patients with RA and 47 healthy volunteers; 27 patients with RA and nine controls were B19+ Cluster of differentiation (CD) 4, 8, 25 and 45RA were analyzed on blood cells. CD25 expression on CD4+CD45RA+, CD4+CD45RA-, CD8+CD45RA+, CD8+CD45RA- subsets were analyzed by flow cytometry. RESULTS: The percentage of CD25low and CD25hi cells was increased on CD4+CD45RA+, CD4+CD45RA- T-cells and the percentage of CD25+ cells was increased on CD8+CD45RA+, CD8+CD45RA- T-cells of B19+ patients with RA in comparison with B19- patients and controls. CONCLUSION: Raised levels of CD4 and CD8 regulatory T-cells in B19+ RA patients could cause down-regulation of antiviral clearance mechanisms and lead to activation of persistent human parvovirus B19 infection in patients with RA. Copyright
AIM: To investigate T-cell subpopulations in peripheral blood of human parvovirus B19 DNA-positive (B19+) and -negative (B19-) patients with rheumatoid arthritis (RA) and healthy persons. PATIENTS AND METHODS: Blood samples were collected from 115 patients with RA and 47 healthy volunteers; 27 patients with RA and nine controls were B19+ Cluster of differentiation (CD) 4, 8, 25 and 45RA were analyzed on blood cells. CD25 expression on CD4+CD45RA+, CD4+CD45RA-, CD8+CD45RA+, CD8+CD45RA- subsets were analyzed by flow cytometry. RESULTS: The percentage of CD25low and CD25hi cells was increased on CD4+CD45RA+, CD4+CD45RA- T-cells and the percentage of CD25+ cells was increased on CD8+CD45RA+, CD8+CD45RA- T-cells of B19+ patients with RA in comparison with B19- patients and controls. CONCLUSION: Raised levels of CD4 and CD8 regulatory T-cells in B19+ RApatients could cause down-regulation of antiviral clearance mechanisms and lead to activation of persistent human parvovirus B19 infection in patients with RA. Copyright
Authors: R Caliskan; S Masatlioglu; M Aslan; S Altun; S Saribas; S Ergin; E Uckan; V Koksal; V Oz; K Altas; I Fresko; B Kocazeybek Journal: Rheumatol Int Date: 2004-08-20 Impact factor: 2.631
Authors: Y Takahashi; C Murai; S Shibata; Y Munakata; T Ishii; K Ishii; T Saitoh; T Sawai; K Sugamura; T Sasaki Journal: Proc Natl Acad Sci U S A Date: 1998-07-07 Impact factor: 11.205
Authors: Gina J Walter; Veerle Fleskens; Klaus S Frederiksen; Megha Rajasekhar; Bina Menon; Jens G Gerwien; Hayley G Evans; Leonie S Taams Journal: Arthritis Rheumatol Date: 2016-01 Impact factor: 10.995