Clerodane Diterpenoid Glucosides from the Stems of Tinospora sinensis.

Ten new clerodane diterpenoid glucosides (1-10) and three known analogues (11-13) were isolated from an EtOAc extract of the stems of Tinospora sinensis. Spectroscopic analyses and chemical methods were used to elucidate the structures of these isolates. The absolute configurations of tinosinenosides A-C (1-3) were established by using experimental and calculated ECD data. Their cytotoxicity against the human epithelioid cervical carcinoma (HeLa) cell line and the nitric oxide production inhibitory activity of lipopolysaccharide-activated N9 microglial cells were tested. 1-Deacetyltinosposide A (12) exhibited mild cytotoxicity against HeLa cells, with an IC50 value of 8.35 ± 0.60 μM.