Histopathological and immunohistochemical studies on nickel sulfide-induced tumors in F344 rats.

Twenty-five tumors induced in F344 male rats were examined histologically and immunohistochemically using antibodies against myoglobin, myosin, desmin and cathepsin B. Eight were from rats which had been given intramuscular (im) injection and 17 were from rats which had been given subcutaneous (sc) injection of 5 mg of Ni3S2. Among 10 rhabdomyosarcomas, myoglobin was detectable in 3, myosin in 8, and desmin in all, but cathepsin B was present in none. Out of 8 malignant fibrous histiocytomas, cathepsin B was detectable in all, but the other antigens were absent. In a leiomyosarcoma, only desmin was detected. In two fibrosarcomas, none of the markers were detected. In four undetermined tumors, one reacted only with anti-desmin antibody, two with only anti-cathepsin B antibody, and one with none of the antibodies. Of the three myogenic markers utilized in this study, anti-desmin antibody appeared to be the most sensitive. Cathepsin B was found mainly in the histiocytic cells of malignant fibrous histiocytoma. Thus, desmin appears to be particularly valuable in distinguishing immature myogenic tumors from other primitive tumors, while cathepsin B is useful in distinguishing malignant fibrous histiocytoma from other pleomorphic mesenchymal tumors.