Masoumeh Ghasemi-Pirbaluti1, Ehsan Motaghi2, Ali Najafi3, Mohammad Javad Hosseini3. 1. Department of Immunology, Shahrekord University of Medical Science, Shahrekord, Iran. 2. Department of Physiology and Pharmacology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj 6618634683, Iran. Electronic address: Motaghi@pharm.mui.ac.ir. 3. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: Ulcerative colitis is a relapsing inflammatory disorder of the colon. There is a need to explore the new treatments for this disorder. Theophylline, a competitive inhibitor of phosphodiesterase, is shown to have anti-inflammatory properties. However, the effect of theophylline on ulcerative colitis has not yet been investigated. The present study evaluated the effect of theophylline on acetic acid induced ulcerative colitis in rats. MATERIALS AND METHODS: Colitis was induced by instillation of 2ml of acetic acid solution (3%). Colon samples were evaluated grossly and microscopically and assayed for myeloperoxidase (MPO) activity and proinflammatory cytokine concentrations. RESULTS: Treatment with theophylline at the doses of 20 and 50mg/kg attenuated acetic acid induced ulcerative colitis as shown by improvement in body weight loss, macroscopic score, ulcer area, hematocrit and histopathological score. Theophylline treatment also reduced MPO activity and tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1 β) and interleukin 6 (IL-6) concentrations in inflamed colon. CONCLUSION: Theophylline has a protective effect in acetic acid-induced ulcerative colitis which might be due to its anti-inflammatory activities. Therefore, theophylline has the potential to be used for successful treatment of ulcerative colitis.
BACKGROUND:Ulcerative colitis is a relapsing inflammatory disorder of the colon. There is a need to explore the new treatments for this disorder. Theophylline, a competitive inhibitor of phosphodiesterase, is shown to have anti-inflammatory properties. However, the effect of theophylline on ulcerative colitis has not yet been investigated. The present study evaluated the effect of theophylline on acetic acid induced ulcerative colitis in rats. MATERIALS AND METHODS:Colitis was induced by instillation of 2ml of acetic acid solution (3%). Colon samples were evaluated grossly and microscopically and assayed for myeloperoxidase (MPO) activity and proinflammatory cytokine concentrations. RESULTS: Treatment with theophylline at the doses of 20 and 50mg/kg attenuated acetic acid induced ulcerative colitis as shown by improvement in body weight loss, macroscopic score, ulcer area, hematocrit and histopathological score. Theophylline treatment also reduced MPO activity and tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1 β) and interleukin 6 (IL-6) concentrations in inflamed colon. CONCLUSION:Theophylline has a protective effect in acetic acid-induced ulcerative colitis which might be due to its anti-inflammatory activities. Therefore, theophylline has the potential to be used for successful treatment of ulcerative colitis.
Authors: Pedro A Ruiz Castro; Ulrike Kogel; Giuseppe Lo Sasso; Blaine W Phillips; Alain Sewer; Bjorn Titz; Llenalia Garcia; Athanasios Kondylis; Emmanuel Guedj; Dariusz Peric; David Bornand; Remi Dulize; Celine Merg; Maica Corciulo; Nikolai V Ivanov; Manuel C Peitsch; Julia Hoeng Journal: J Inflamm (Lond) Date: 2020-08-24 Impact factor: 4.981