Barbara Kofler1, Wegene Borena2, Claudia Manzl3, Jozsef Dudas4, Anne-Sophie Wegscheider3, Pidder Jansen-Dürr5, Volker Schartinger4, Herbert Riechelmann4. 1. Department of Otorhinolaryngology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria. Electronic address: ba.kofler@tirol-kliniken.at. 2. Division of Virology, Department of Hygiene, Microbiology, Social Medicine, Medical University of Innsbruck, Peter-Mayr-Strasse 4b, 6020 Innsbruck, Austria. 3. Department of Pathology, Medical University of Innsbruck, Müllerstrasse 44, 6020 Innsbruck, Austria. 4. Department of Otorhinolaryngology, Medical University of Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria. 5. Institute for Biomedical Ageing Research, Medical University of Innsbruck, Rennweg 10, 6020 Innsbruck, Austria.
Abstract
OBJECTIVE: Human papilloma virus (HPV) induced head and neck squamous cell carcinoma (HNSCC) represents a distinct tumor subset. We questioned how accurately a brushing from the tumor surface detects HPV in patients with HNSCC. MATERIALS AND METHODS: Brushings from the tumor surface were compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) tumor biopsies, which served as the reference standard. In both matrices, HPV DNA was detected using a commercially available test kit. In addition, p16 was assessed in tumor biopsies by immunohistochemistry (IHC). The tumors were considered p16 positive if 70% or more of cancer cells expressed p16. RESULTS: 93 patients with HNSCC were included. Sensitivity and specificity of the brush test were 83% (95%CI: 67-92%) and 85% (95%CI: 72-93%). Results of p16 IHC were concordant with FFPE samples DNA determinations in 73/93 patients. In 53 patients (57%) the tumor was located in the oropharynx and in 40 patients (43%) the tumor was located in the non-oropharynx region. Sensitivity and specificity of the brush test in patients with oropharyngeal cancer was higher with 86% (95%CI: 70-95%) and 89% (95%CI: 65-99%). CONCLUSION: Superficial brushes from the tumor surface may be used to identify HPV positive HNSCC.
OBJECTIVE:Human papilloma virus (HPV) induced head and neck squamous cell carcinoma (HNSCC) represents a distinct tumor subset. We questioned how accurately a brushing from the tumor surface detects HPV in patients with HNSCC. MATERIALS AND METHODS: Brushings from the tumor surface were compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) tumor biopsies, which served as the reference standard. In both matrices, HPV DNA was detected using a commercially available test kit. In addition, p16 was assessed in tumor biopsies by immunohistochemistry (IHC). The tumors were considered p16 positive if 70% or more of cancer cells expressed p16. RESULTS: 93 patients with HNSCC were included. Sensitivity and specificity of the brush test were 83% (95%CI: 67-92%) and 85% (95%CI: 72-93%). Results of p16 IHC were concordant with FFPE samples DNA determinations in 73/93 patients. In 53 patients (57%) the tumor was located in the oropharynx and in 40 patients (43%) the tumor was located in the non-oropharynx region. Sensitivity and specificity of the brush test in patients with oropharyngeal cancer was higher with 86% (95%CI: 70-95%) and 89% (95%CI: 65-99%). CONCLUSION: Superficial brushes from the tumor surface may be used to identify HPV positive HNSCC.
Authors: Teresa Bernadette Steinbichler; L Golm; D Dejaco; D Riedl; B Kofler; C Url; D Wolfram; H Riechelmann Journal: Eur Arch Otorhinolaryngol Date: 2020-01-25 Impact factor: 2.503
Authors: József Dudás; Wolfgang Dietl; Angela Romani; Susanne Reinold; Rudolf Glueckert; Anneliese Schrott-Fischer; Daniel Dejaco; Lejo Johnson Chacko; Raphaela Tuertscher; Volker Hans Schartinger; Herbert Riechelmann Journal: Int J Mol Sci Date: 2018-06-14 Impact factor: 5.923