| Literature DB >> 2835103 |
M Esmann1.
Abstract
A method for preparation of membranous fragments of pure and highly active shark rectal gland Na+/K+-ATPase by Mn2+ precipitation of C12E8-solubilized enzyme is described. The method is rapid and inexpensive, and yields enzyme with a specific Na+/K+-ATPase activity of up to 1800 mumol/mg per h at 37 degrees C. The influence of the detergent/protein and lipid/protein ratios on the yield of membrane bound enzyme is described.Entities:
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Year: 1988 PMID： 2835103 DOI： 10.1016/0005-2736(88)90009-0
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002