| Literature DB >> 28349460 |
Abstract
For the transcriptome-wide detection and quantification of the 5-methylcytosine (m5C) methylation modification of RNA, one experimental approach is via bisulfite conversion. In this chapter we discuss statistical methods, and a corresponding computational pipeline, to perform transcriptome-wide differential m5C methylation analysis between RNA samples, specialized for this assay.Entities:
Keywords: 5-methylcytosine; Bisulfite conversion; Differential methylation; Epitranscriptomics; High-throughput sequencing; RNA methylation
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Year: 2017 PMID： 28349460 DOI： 10.1007/978-1-4939-6807-7_11
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745