| Literature DB >> 28347471 |
Alaa El-Din Hamid Sayed1, Kento Igarashi2, Tomomi Watanabe-Asaka2, Hiroshi Mitani2.
Abstract
The study of the DNA damage response in erythrocytes after γ-irradiation may provide evidence for its effectiveness as a biomarkers for genotoxic environmental stress. We previously reported various malformations in erythrocytes of medaka irradiated with10 Gy, but not in their micronuclei. In this study, we optimized an assay method for γ-H2AX and double strand breaks in erythrocytes of adult medaka fish after 15 Gy of γ-irradiation. The highest level of apoptosis and nuclear abnormalities, including in micronuclei, were recorded 4 h after γ-irradiation, as was the highest level of γ-H2AX foci in erythrocytes. These results suggest that recognition and repair processes occur as a response to DNA damage in erythrocytes in medaka.Entities:
Keywords: DNA repair; Erythrocytes; Fish; Radiation; γ-H2AX
Mesh：
Substances：
Year: 2017 PMID： 28347471 DOI： 10.1016/j.envpol.2016.11.050
Source DB: PubMed Journal: Environ Pollut ISSN： 0269-7491 Impact factor: 8.071