Rüdiger M Zimmerer1, Nils Ludwig2, Andreas Kampmann2, Gido Bittermann3, Simon Spalthoff2, Michael Jungheim4, Nils-Claudius Gellrich2, Frank Tavassol2. 1. Department of Oral and Maxillofacial Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. Electronic address: zimmerer.ruediger@mh-hannover.de. 2. Department of Oral and Maxillofacial Surgery, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany. 3. Department of Oral and Maxillofacial Surgery, University of Freiburg Medical School, Hugstetter-Str. 55, 79104 Freiburg, Germany. 4. Department of Phoniatrics and Pediatric Audiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.
Abstract
BACKGROUND: In oral squamous cell carcinoma (OSCC), a minor subset of cancer stem cells has been identified using the surface marker CD24. The CD24+ cell population is involved in initiating, maintaining, and expanding tumor growth, but has not been reported to be involved in angiogenesis to date. METHODS: NOD/SCID mice were equipped with dorsal skinfold chambers and gelatin sponges seeded with CD24+, CD24-, and unsorted cancer cells suspended in Matrigel® were implanted. Following intravital fluorescence microscopy, specimens were examined by immunohistology. RESULTS: Sponges seeded with CD24+ cells showed a significantly higher functional capillary density than those seeded with CD24- cells. The presence of endothelial cells was confirmed by immunohistochemistry for CD31. CONCLUSION: For the first time, CD24+ tumorigenic cells with angiogenic potential, which were isolated from OSCC, were characterized. Our findings provide a promising in vivo model to facilitate the development of therapeutic agents against cancer stem cells and their angiogenic pathways.
BACKGROUND: In oral squamous cell carcinoma (OSCC), a minor subset of cancer stem cells has been identified using the surface marker CD24. The CD24+ cell population is involved in initiating, maintaining, and expanding tumor growth, but has not been reported to be involved in angiogenesis to date. METHODS: NOD/SCIDmice were equipped with dorsal skinfold chambers and gelatin sponges seeded with CD24+, CD24-, and unsorted cancer cells suspended in Matrigel® were implanted. Following intravital fluorescence microscopy, specimens were examined by immunohistology. RESULTS: Sponges seeded with CD24+ cells showed a significantly higher functional capillary density than those seeded with CD24- cells. The presence of endothelial cells was confirmed by immunohistochemistry for CD31. CONCLUSION: For the first time, CD24+ tumorigenic cells with angiogenic potential, which were isolated from OSCC, were characterized. Our findings provide a promising in vivo model to facilitate the development of therapeutic agents against cancer stem cells and their angiogenic pathways.
Authors: Sarah Gniesmer; Ralph Brehm; Andrea Hoffmann; Dominik de Cassan; Henning Menzel; Anna-Lena Hoheisel; Birgit Glasmacher; Elmar Willbold; Janin Reifenrath; Mathias Wellmann; Nils Ludwig; Frank Tavassol; Ruediger Zimmerer; Nils-Claudius Gellrich; Andreas Kampmann Journal: J Tissue Eng Regen Med Date: 2019-05-29 Impact factor: 3.963
Authors: Sarah Gniesmer; Ralph Brehm; Andrea Hoffmann; Dominik de Cassan; Henning Menzel; Anna Lena Hoheisel; Birgit Glasmacher; Elmar Willbold; Janin Reifenrath; Nils Ludwig; Ruediger Zimmerer; Frank Tavassol; Nils-Claudius Gellrich; Andreas Kampmann Journal: PLoS One Date: 2020-01-13 Impact factor: 3.240