Literature DB >> 2834350

A rapid vapor-phase acid (hydrochloric acid and trifluoroacetic acid) hydrolysis of peptide and protein.

A Tsugita1, T Uchida, H W Mewes, T Ataka.   

Abstract

A new method for the acid hydrolysis of protein is presented. Peptide bonds are cleaved by the action of an HCl/trifluoroacetic acid (TFA) vapor mixture. Contamination for the hydrolysis mixture is reduced to low levels (1-3 pmol). Recovery of hydrophobic amino acid is improved. Short reaction times are achieved and rapid removal of acids is facilitated. The reaction temperature is 158 degrees C for reaction times of 22.5 and 45 min with 7 M HCl and 10% TFA containing 0.1% phenol.

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Year:  1987        PMID： 2834350     DOI： 10.1093/oxfordjournals.jbchem.a122209

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  12 in total

1.  Immunochemical studies and complete amino acid sequence of the streptokinase from Streptococcus pyogenes (group A) M type 12 strain A374.

Authors:  H Ohkuni; Y Todome; H Suzuki; M Mizuse; N Kotani; K Horiuchi; N Shikama; A Tsugita; K H Johnston
Journal:  Infect Immun       Date:  1992-01       Impact factor: 3.441

2.  Nucleotide and derived amino acid sequences of the major porin of Comamonas acidovorans and comparison of porin primary structures.

Authors:  S Gerbl-Rieger; J Peters; J Kellermann; F Lottspeich; W Baumeister
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

3.  Unusual amino acid sequence of fasciatoxin, a weak reversibly acting neurotoxin in the venom of the banded krait, Bungarus fasciatus.

Authors:  C S Liu; P W Hsiao; C S Chang; M C Tzeng; T B Lo
Journal:  Biochem J       Date:  1989-04-01       Impact factor: 3.857

4.  Rapid identification of proteins.

Authors:  G Shaw
Journal:  Proc Natl Acad Sci U S A       Date:  1993-06-01       Impact factor: 11.205

5.  Amino acids in the Martian meteorite Nakhla.

Authors:  D P Glavin; J L Bada; K L Brinton; G D McDonald
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

6.  Structure and biological activities of a new mastoparan isolated from the venom of the hornet Vespa basalis.

Authors:  C L Ho; L L Hwang
Journal:  Biochem J       Date:  1991-03-01       Impact factor: 3.857

7.  Primary structure and characterization of an androgen-stimulated autoantigen purified from mouse seminal-vesicle secretion.

Authors:  L C Yu; J L Chen; W B Tsai; Y H Chen
Journal:  Biochem J       Date:  1993-12-15       Impact factor: 3.857

8.  Determination of the structure of two novel echistatin variants and comparison of the ability of echistatin variants to inhibit aggregation of platelets from different species.

Authors:  Y L Chen; T F Huang; S W Chen; I H Tsai
Journal:  Biochem J       Date:  1995-01-15       Impact factor: 3.857

9.  S-layer protein gene of Acetogenium kivui: cloning and expression in Escherichia coli and determination of the nucleotide sequence.

Authors:  J Peters; M Peters; F Lottspeich; W Baumeister
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

10.  Primary structure of sensory rhodopsin I, a prokaryotic photoreceptor.

Authors:  A Blanck; D Oesterhelt; E Ferrando; E S Schegk; F Lottspeich
Journal:  EMBO J       Date:  1989-12-20       Impact factor: 11.598
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