A Armentia1, J Santos2, Z Serrano3, B Martín3, S Martín4, J Barrio2, S Fernández4, M González-Sagrado5, F Pineda6, R Palacios6. 1. Servicio de Alergia, Hospital Universitario Río Hortega, Spain. Electronic address: aliciaarmentia@gmail.com. 2. Servicio de Gastroenterología, Hospital Universitario Río Hortega, Spain. 3. Servicio de Alergia, Hospital Universitario Río Hortega, Spain. 4. Servicio de Pediatría, Hospital Universitario Río Hortega, Spain. 5. Unidad de apoyo a la Investigación, Hospital Universitario Río Hortega, Spain. 6. Laboratorios Diater, Madrid, Spain.
Abstract
BACKGROUND: There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied. OBJECTIVE: To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shellfish or cephalopods, compared with healthy subjects, pollen allergic individuals and children with digestive symptoms after eating marine food. METHODS: We carried out in vivo tests (skin prick) and in vitro tests (specific IgE determination, Western blot) and component resolved diagnostics (CRD) using microarray analysis in all patients. RESULTS: CRD better detected sensitisation to allergens from marine parasites than skin prick tests and determination of specific IgE by CAP. Sensitisation to Gymnorhynchus gigas was detected in 26% of patients measured by skin prick tests and 36% measured by IgE. CONCLUSIONS: The prevalence of hypersensitivity to marine parasite allergens other than Anisakis simplex should be studied, and the most appropriate technique for this is CRD.
BACKGROUND: There has been an increase in the prevalence of hypersensitivity to Anisakis simplex. There are fish parasites other than Anisakis simplex whose allergenicity has not yet been studied. OBJECTIVE: To assess IgE hypersensitivity caused by fish parasite allergens in patients with gastro-allergic symptoms after consumption of fish, shellfish or cephalopods, compared with healthy subjects, pollen allergic individuals and children with digestive symptoms after eating marine food. METHODS: We carried out in vivo tests (skin prick) and in vitro tests (specific IgE determination, Western blot) and component resolved diagnostics (CRD) using microarray analysis in all patients. RESULTS: CRD better detected sensitisation to allergens from marine parasites than skin prick tests and determination of specific IgE by CAP. Sensitisation to Gymnorhynchus gigas was detected in 26% of patients measured by skin prick tests and 36% measured by IgE. CONCLUSIONS: The prevalence of hypersensitivity to marine parasite allergens other than Anisakis simplex should be studied, and the most appropriate technique for this is CRD.
Authors: Maciej Kochanowski; Mirosław Różycki; Joanna Dąbrowska; Aneta Bełcik; Jacek Karamon; Jacek Sroka; Tomasz Cencek Journal: Biomolecules Date: 2020-07-16