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Literature DB >> 2834096

Curing of endogenous 2 micron DNA in yeast by recombinant vectors.

Abstract

Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining cir degree derivatives of any yeast strain.

Entities: Gene Species

Mesh：

Substances：

Year: 1987 PMID： 2834096 DOI： 10.1007/BF00355406

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

12 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Replication and recombination functions associated with the yeast plasmid, 2 mu circle.

Authors: J R Broach; J B Hicks
Journal: Cell Date: 1980-09 Impact factor: 41.582

3. Transformation of yeast by a replicating hybrid plasmid.

Authors: J D Beggs
Journal: Nature Date: 1978-09-14 Impact factor: 49.962

4. Isolation of the yeast regulatory gene GAL4 and analysis of its dosage effects on the galactose/melibiose regulon.

Authors: S A Johnston; J E Hopper
Journal: Proc Natl Acad Sci U S A Date: 1982-11 Impact factor: 11.205

5. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

6. Curing of Saccharomyces cerevisiae 2-μm DNA by transformation.

Authors: E Erhart; C P Hollenberg
Journal: Curr Genet Date: 1981-05 Impact factor: 3.886

7. Stability of a cloned gene in yeast grown in chemostat culture.

Authors: R M Walmsley; D C Gardner; S G Oliver
Journal: Mol Gen Genet Date: 1983

8. The presence of a defective LEU2 gene on 2 mu DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number.

Authors: E Erhart; C P Hollenberg
Journal: J Bacteriol Date: 1983-11 Impact factor: 3.490

9. Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.

Authors: M J Dobson; A B Futcher; B S Cox
Journal: Curr Genet Date: 1980-12 Impact factor: 3.886

10. Properties of REP3: a cis-acting locus required for stable propagation of the Saccharomyces cerevisiae plasmid 2 microns circle.

Authors: M Jayaram; A Sutton; J R Broach
Journal: Mol Cell Biol Date: 1985-09 Impact factor: 4.272

6 in total

1. When a glycolytic gene on a yeast 2 mu ORI-STB plasmid is made essential for growth its expression level is a major determinant of plasmid copy number.

Authors: P W Piper; B P Curran
Journal: Curr Genet Date: 1990-02 Impact factor: 3.886

5. FLP-FRT mediated intrachromosomal recombination on a tandemly duplicated YEp integrant at the ILV2 locus of chromosome XIII in Saccharomyces cerevisiae.

Authors: G H Rank; G M Arndt; W Xiao
Journal: Curr Genet Date: 1989-02 Impact factor: 3.886

6. Polymorphism within the nuclear and 2 micron genomes of Saccharomyces cerevisiae.

Authors: G H Rank; G P Casey; W Xiao; A T Pringle
Journal: Curr Genet Date: 1991-08 Impact factor: 3.886