Marian Garcia-Nuñez1,2,3,4, Sara Marti1,5, Carmen Puig1,5, Vicente Perez-Brocal6,7,8, Laura Millares1,2,3,4, Salud Santos1,9, Carmen Ardanuy1,5, Andres Moya6,7,8, Josefina Liñares1,5, Eduard Monsó1,2,3,4. 1. CIBER de Enfermedades Respiratorias (CIBERes), Instituto de Salud Carlos III, Madrid, Spain E-28029. 2. Department of Respiratory Medicine, Parc Taulí Hospital Universitari. Institut d'Investigació i Innovació Parc Taulí I3PT, Sabadell, Spain E-08208. 3. Fundació Institut d'Investigació Germans Trias i Pujol, Badalona, Spain E-08916. 4. Universitat Autonoma de Barcelona, Esfera UAB, Barcelona, Spain E-08193. 5. Microbiology Department, Hospital Universitari Bellvitge, Universitat de Barcelona-IDIBELL, Barcelona, Spain E-08908. 6. Genomics & Health Area, Centro Superior de Investigación en Salud Pública - Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (CSISP-FISABIO), Valencia, Spain E-46020. 7. Department of Genetics, Institut Cavanilles de Biodiversitat i Biologia Evolutiva, (ICBiBE) Universitat de València, València, Spain E-46020. 8. CIBER Epidemiología y Salud Pública (CIBERESP), Instituto de Salud Carlos III, Madrid, Spain E-28029. 9. Department of Pulmonary Medicine, Hospital Universitari de Bellvitge, Institut d'Investigacions Biomèdiques de Bellvitge (IDIBELL), Universitat de Barcelona, Barcelona, Spain E-08908.
Abstract
AIM: The bronchial microbiome of severe chronic obstructive pulmonary disease patients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity. PATIENTS & METHODS: Patient sputum samples from 21 patients were studied. RESULTS: Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances <1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances >50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven. CONCLUSION: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.
AIM: The bronchial microbiome of severe chronic obstructive pulmonary diseasepatients colonized by Pseudomonas aeruginosa was analyzed using 16S rRNA gene sequencing to identify differences related to biofilm-forming capacity. PATIENTS & METHODS:Patient sputum samples from 21 patients were studied. RESULTS: Statistically significant differences related to biofilm-forming capacity were only found for genera with relative abundances <1%, and Fusobacterium was over-represented when biofilm-forming capacity was high. Genera with relative abundances >50% which increased from baseline were observed in 10/14 exacerbations, but corresponded to Pseudomonas only in three episodes, while other pathogenic genera were identified in seven. CONCLUSION: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary diseasepatients.
Entities:
Keywords:
16S rRNA; P. aeruginosa; biofilm; exacerbation; lung microbiome; severe COPD
Authors: Sebastián A Riquelme; Kalle Liimatta; Tania Wong Fok Lung; Blanche Fields; Danielle Ahn; David Chen; Carmen Lozano; Yolanda Sáenz; Anne-Catrin Uhlemann; Barbara C Kahl; Clemente J Britto; Emily DiMango; Alice Prince Journal: Cell Metab Date: 2020-05-18 Impact factor: 27.287
Authors: Laia Fernández-Barat; Ana Motos; Otavio T Ranzani; Gianluigi Li Bassi; Elisabet Aguilera Xiol; Tarek Senussi; Chiara Travierso; Chiara Chiurazzi; Francesco Idone; Laura Muñoz; Jordi Vila; Miquel Ferrer; Paolo Pelosi; Francesco Blasi; Massimo Antonelli; Antoni Torres Journal: Microorganisms Date: 2017-09-20