Literature DB >> 28337708

Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.

Yun Chen1,2, Chuan Li3, Ya-Hui Tsai4,5, Sheng-Hong Tseng6.   

Abstract

The isolation and culture of intestinal stem cells (ISCs) was first demonstrated in the very recent decade with the identification of ISC marker Lgr5. The growth of ISCs into crypt organoids provides an in vitro model for studying the mucosal physiology, intestinal cancer tumorigenesis, and intestinal regeneration. Here, we describe two different isolation protocols and demonstrate a fixation method that aids in the confocal observation of the organoids.

Entities:  

Keywords:  Confocal observation; Crypt organoid; Intestinal stem cell (ISC); Isolation; Three-dimensional imaging

Mesh：

Year:  2019        PMID： 28337708     DOI： 10.1007/7651_2017_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

1.  Repressed E-cadherin expression in the lower crypt of human small intestine: a cell marker of functional relevance.

Authors:  Fabrice Escaffit; Nathalie Perreault; Dominique Jean; Caroline Francoeur; Elizabeth Herring; Claudine Rancourt; Nathalie Rivard; Pierre H Vachon; Frédéric Paré; Marie-Pierre Boucher; Joëlle Auclair; Jean-François Beaulieu
Journal:  Exp Cell Res       Date:  2005-01-15       Impact factor: 3.905

Review 2.  Growing self-organizing mini-guts from a single intestinal stem cell: mechanism and applications.

Authors:  Toshiro Sato; Hans Clevers
Journal:  Science       Date:  2013-06-07       Impact factor: 47.728

3.  Ischemic preconditioning increased the intestinal stem cell activities in the intestinal crypts in mice.

Authors:  Yun Chen; Shih-Hua Lee; Ya-Hui Tsai; Sheng-Hong Tseng
Journal:  J Surg Res       Date:  2013-10-10       Impact factor: 2.192

4.  Intestinal stem cells and stem cell-based therapy for intestinal diseases.

Authors:  Mahmoud Shaaban Mohamed; Yun Chen; Chao-Ling Yao
Journal:  Cytotechnology       Date:  2014-07-01       Impact factor: 2.058

5.  LPS receptor subunits have antagonistic roles in epithelial apoptosis and colonic carcinogenesis.

Authors:  W-T Kuo; T-C Lee; H-Y Yang; C-Y Chen; Y-C Au; Y-Z Lu; L-L Wu; S-C Wei; Y-H Ni; B-R Lin; Y Chen; Y-H Tsai; J T Kung; F Sheu; L-W Lin; L C-H Yu
Journal:  Cell Death Differ       Date:  2015-01-30       Impact factor: 15.828

6.  Proinflammatory cytokines TNF-alpha and IFN-gamma alter laminin expression under an apoptosis-independent mechanism in human intestinal epithelial cells.

Authors:  Caroline Francoeur; Fabrice Escaffit; Pierre H Vachon; Jean-François Beaulieu
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2004-04-15       Impact factor: 4.052

7.  Oligosaccharides from human milk influence growth-related characteristics of intestinally transformed and non-transformed intestinal cells.

Authors:  Sabine Kuntz; Silvia Rudloff; Clemens Kunz
Journal:  Br J Nutr       Date:  2007-10-10       Impact factor: 3.718

8.  Application of three-dimensional imaging to the intestinal crypt organoids and biopsied intestinal tissues.

Authors:  Yun Chen; Ya-Hui Tsai; Yuan-An Liu; Shih-Hua Lee; Sheng-Hong Tseng; Shiue-Cheng Tang
Journal:  ScientificWorldJournal       Date:  2013-11-14

9.  Autologous transplantation of intestine-isolated glia cells improves neuropathology and restores cognitive deficits in β amyloid-induced neurodegeneration.

Authors:  Giuseppe Esposito; Giovanni Sarnelli; Elena Capoccia; Carla Cirillo; Marcella Pesce; Jie Lu; Gaetano Calì; Rosario Cuomo; Luca Steardo
Journal:  Sci Rep       Date:  2016-03-04       Impact factor: 4.379

10.  Development of Functional Microfold (M) Cells from Intestinal Stem Cells in Primary Human Enteroids.

Authors:  Joshua D Rouch; Andrew Scott; Nan Ye Lei; R Sergio Solorzano-Vargas; Jiafang Wang; Elaine M Hanson; Masae Kobayashi; Michael Lewis; Matthias G Stelzner; James C Y Dunn; Lars Eckmann; Martín G Martín
Journal:  PLoS One       Date:  2016-01-28       Impact factor: 3.752
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  2 in total

Review 1.  Patient-derived organoid models help define personalized management of gastrointestinal cancer.

Authors:  M R Aberle; R A Burkhart; H Tiriac; S W M Olde Damink; C H C Dejong; D A Tuveson; R M van Dam
Journal:  Br J Surg       Date:  2018-01       Impact factor: 6.939

Review 2.  Influence of Growth Hormone and Glutamine on Intestinal Stem Cells: A Narrative Review.

Authors:  Yun Chen; Ya-Hui Tsai; Bor-Jiun Tseng; Sheng-Hong Tseng
Journal:  Nutrients       Date:  2019-08-17       Impact factor: 5.717
  2 in total

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