Manuela Morato1,2,3,4, Liane Correia-Costa5,6,7, Teresa Sousa8,9, Dina Cosme8,5, Franz Schaefer10, José Carlos Areias11, António Guerra7,12,13, Alberto Caldas Afonso5,6,7, Henrique Barros5,14, Ana Azevedo5,14, António Albino-Teixeira8,9. 1. Department of Pharmacology and Therapeutics, Faculty of Medicine of University of Porto, Porto, Portugal. mmorato@ff.up.pt. 2. Department of Drug Sciences, Laboratory of Pharmacology, Faculty of Pharmacy of the University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal. mmorato@ff.up.pt. 3. MedInUP - Center for Drug Discovery and Innovative Medicines, University of Porto, Porto, Portugal. mmorato@ff.up.pt. 4. Laboratory of Pharmacology, Department of Drug Sciences, Faculty of Pharmacy of University of Porto, Porto, Portugal. mmorato@ff.up.pt. 5. EPIUnit - Institute of Public Health, University of Porto, Porto, Portugal. 6. Division of Pediatric Nephrology, Integrated Pediatric Hospital, Centro Hospitalar São João, Porto, Portugal. 7. Department of Pediatrics, Faculty of Medicine of University of Porto, Porto, Portugal. 8. Department of Pharmacology and Therapeutics, Faculty of Medicine of University of Porto, Porto, Portugal. 9. MedInUP - Center for Drug Discovery and Innovative Medicines, University of Porto, Porto, Portugal. 10. Division of Pediatric Nephrology, Center for Pediatrics and Adolescent Medicine, University of Heidelberg, Heidelberg, Germany. 11. Division of Pediatric Cardiology, Integrated Pediatric Hospital, Centro Hospitalar São João, Porto, Portugal. 12. Division of Pediatric Nutrition, Integrated Pediatric Hospital, Centro Hospitalar São João, Porto, Portugal. 13. CINTESIS - Center for Research in Health Technologies and Information Systems, Faculty of Medicine, University of Porto, Porto, Portugal. 14. Department of Clinical Epidemiology, Predictive Medicine and Public Health, Faculty of Medicine of University of Porto, Porto, Portugal.
Abstract
BACKGROUND: We aimed to study the impact of obesity on urinary excretion of angiotensinogen (U-AGT) in prepubertal children, focusing on the duration of obesity and gender. Also, we aimed to evaluate whether plasma angiotensinogen (P-AGT) and hydrogen peroxide (H2O2) play a role in the putative association. METHODS: Cross-sectional evaluation of 305 children aged 8-9 years (160 normal weight, 86 overweight, and 59 obese). Anthropometric measurements and 24-h ambulatory blood pressure monitoring were performed. Angiotensinogen (AGT) was determined by a commercial enzyme-linked immunosorbent assay (ELISA) kit and H2O2 by a microplate fluorometric assay. RESULTS: U-AGT and P-AGT levels were similar across body mass index (BMI) groups and between sexes. However, boys who were overweight/obese since the age of 4 years presented lower levels of U-AGT compared with those of normal weight at the same age. In children who were overweight/obese since the age of 4, urinary H2O2 decreased with P-AGT. CONCLUSIONS: A higher duration of obesity was associated with decreased U-AGT in boys, thus reflecting decreased intrarenal activity of the renin-angiotensin system. Also, children with a longer duration of obesity showed an inverse association between urinary H2O2 and P-AGT. Future studies should address whether these results reflect an early compensatory mechanism to limit obesity-triggered renal dysfunction.
BACKGROUND: We aimed to study the impact of obesity on urinary excretion of angiotensinogen (U-AGT) in prepubertal children, focusing on the duration of obesity and gender. Also, we aimed to evaluate whether plasma angiotensinogen (P-AGT) and hydrogen peroxide (H2O2) play a role in the putative association. METHODS: Cross-sectional evaluation of 305 children aged 8-9 years (160 normal weight, 86 overweight, and 59 obese). Anthropometric measurements and 24-h ambulatory blood pressure monitoring were performed. Angiotensinogen (AGT) was determined by a commercial enzyme-linked immunosorbent assay (ELISA) kit and H2O2 by a microplate fluorometric assay. RESULTS: U-AGT and P-AGT levels were similar across body mass index (BMI) groups and between sexes. However, boys who were overweight/obese since the age of 4 years presented lower levels of U-AGT compared with those of normal weight at the same age. In children who were overweight/obese since the age of 4, urinary H2O2 decreased with P-AGT. CONCLUSIONS: A higher duration of obesity was associated with decreased U-AGT in boys, thus reflecting decreased intrarenal activity of the renin-angiotensin system. Also, children with a longer duration of obesity showed an inverse association between urinary H2O2 and P-AGT. Future studies should address whether these results reflect an early compensatory mechanism to limit obesity-triggered renal dysfunction.
Entities:
Keywords:
Angiotensinogen; Body mass index; Children; Hydrogen peroxide; Obesity; Overweight
Authors: M Barton; R Carmona; H Morawietz; L V d'Uscio; W Goettsch; H Hillen; C C Haudenschild; J E Krieger; K Münter; T Lattmann; T F Lüscher; S Shaw Journal: Hypertension Date: 2000-01 Impact factor: 10.190
Authors: T Sousa; S Oliveira; J Afonso; M Morato; D Patinha; S Fraga; F Carvalho; A Albino-Teixeira Journal: Br J Pharmacol Date: 2012-08 Impact factor: 8.739
Authors: M-L Brezniceanu; F Liu; C-C Wei; S Tran; S Sachetelli; S-L Zhang; D-F Guo; J G Filep; J R Ingelfinger; J S D Chan Journal: Kidney Int Date: 2007-03-07 Impact factor: 10.612