Literature DB >> 28337277

Effect and mechanism of SHED on ulcer wound healing in Sprague-Dawley rat models with diabetic ulcer.

Yue Lv1, Lihong Ge1, Yuming Zhao1.   

Abstract

To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) upon the ulcer wound healing and evaluate the mechanism underlying the role of SHED in Sprague-Dawley rat models with diabetic foot ulcer. The rats with diabetic ulcer were established and treated with SHED, mesenchymal stem cell (MSC) and PBS, respectively. The expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), matrix metalloproteinase-2 (MMP-2) and MMP9 at both protein and RNA levels was quantitatively measured. The serum levels of VEGF, IL-1β, TNF-1α and IL-10 were detected by ELISA. The remaining tissues were fixed in 4% chloral hydrate for hematoxylin and eosin (H.E) staining and immunohistochemical staining. MSC and SHED administration could reduce ulceration area and accelerate wound healing at 7 and 14 d after treatment as compared with the control group (all P<0.05), which were validated by H.E and immunohistochemical staining. Western blot results revealed that the expression levels of VEGF, eNOS, MMP2 and MMP9 proteins in the MSC and SHED groups were considerably up-regulated compared with those in the control group at different time points (all P<0.05). The same trend was also observed in the mRNA expression of these cytokines detected by RT-PCR. At 3-d after treatment, no statistical significance was noted in the IL-10 level among three groups, but the IL-10 concentration in the SHED and MSC groups was significantly down-regulated at 7- and 14-d post-treatment (all P<0.05). SHED administration, similar to MSCs, could accelerate wound healing, promote angiogenesis and suppress inflammatory responses in rat models with diabetic ulceration.

Entities:  

Keywords:  SHED; cytokine; diabetic ulcer; mesenchymal stem cell

Year:  2017        PMID: 28337277      PMCID: PMC5340684     

Source DB:  PubMed          Journal:  Am J Transl Res            Impact factor:   4.060


  24 in total

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2.  Potential characteristics of stem cells from human exfoliated deciduous teeth compared with bone marrow-derived mesenchymal stem cells for mineralized tissue-forming cell biology.

Authors:  Kenji Hara; Yoichi Yamada; Sayaka Nakamura; Eri Umemura; Kenji Ito; Minoru Ueda
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Authors:  Margaret K Strecker-McGraw; Thomas Russel Jones; David G Baer
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Authors:  Haihong Li; Xiaobing Fu; Yunshu Ouyang; Cunliang Cai; Jun Wang; Tongzhu Sun
Journal:  Cell Tissue Res       Date:  2006-08-12       Impact factor: 5.249

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Review 7.  Advanced glycoxidation products and impaired diabetic wound healing.

Authors:  Melpomeni Peppa; Panagiotis Stavroulakis; Sotirios A Raptis
Journal:  Wound Repair Regen       Date:  2009 Jul-Aug       Impact factor: 3.617

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10.  Therapeutic Potential of Stem Cells from Human Exfoliated Deciduous Teeth in Models of Acute Kidney Injury.

Authors:  Yuka Hattori; Hangsoo Kim; Naotake Tsuboi; Akihito Yamamoto; Shinichi Akiyama; Yiqin Shi; Takayuki Katsuno; Tomoki Kosugi; Minoru Ueda; Seiichi Matsuo; Shoichi Maruyama
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1.  Expression of miR-217 and HIF-1α/VEGF pathway in patients with diabetic foot ulcer and its effect on angiogenesis of diabetic foot ulcer rats.

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Journal:  J Endocrinol Invest       Date:  2019-05-11       Impact factor: 4.256

2.  Stem Cells from Human Exfoliated Deciduous Teeth Ameliorate Diabetic Nephropathy In Vivo and In Vitro by Inhibiting Advanced Glycation End Product-Activated Epithelial-Mesenchymal Transition.

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Review 3.  Stem Cell-Based Therapy for Diabetic Foot Ulcers.

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Journal:  Front Cell Dev Biol       Date:  2022-02-01

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Review 5.  Stem cell therapy for diabetic foot ulcers: a review of preclinical and clinical research.

Authors:  Lara Lopes; Ocean Setia; Afsha Aurshina; Shirley Liu; Haidi Hu; Toshihiko Isaji; Haiyang Liu; Tun Wang; Shun Ono; Xiangjiang Guo; Bogdan Yatsula; Jianming Guo; Yongquan Gu; Tulio Navarro; Alan Dardik
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6.  The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p.

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7.  Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis.

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