J M S Loh1, J-Y C Tsai2, T Proft2. 1. Department of Molecular Medicine & Pathology, School of Medical Sciences, University of Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, New Zealand. Electronic address: mj.loh@auckland.ac.nz. 2. Department of Molecular Medicine & Pathology, School of Medical Sciences, University of Auckland, New Zealand; Maurice Wilkins Centre for Molecular Biodiscovery, University of Auckland, New Zealand.
Abstract
OBJECTIVES: To determine if emm pattern-inferred tissue tropisms of Group A streptococcus strains is reflected in their ability to adhere to immortalized human HaCat (keratinocyte) and Detroit 562 (pharyngeal) cell lines. METHODS: Human epithelial cell line monolayers were inoculated with Group A streptococcus, and the percentage of adhered bacteria after a 1-h incubation period was calculated. RESULTS: Of the 21/69 inferred-skin-tropic, and the 25/69 inferred-throat-tropic isolates no preferential adherence was observed to a particular cell line. The 23/69 strains classified as 'generalists', however, showed an overall greater ability to adhere to both cell lines. CONCLUSIONS: Predicted tissue-tropism based on emm patterns is not reflected by preferential adherence to a specific cell line, suggesting that early adhesion events may not be as important in establishing infection at a particular ecological niche than originally expected.
OBJECTIVES: To determine if emm pattern-inferred tissue tropisms of Group A streptococcus strains is reflected in their ability to adhere to immortalized human HaCat (keratinocyte) and Detroit 562 (pharyngeal) cell lines. METHODS:Human epithelial cell line monolayers were inoculated with Group A streptococcus, and the percentage of adhered bacteria after a 1-h incubation period was calculated. RESULTS: Of the 21/69 inferred-skin-tropic, and the 25/69 inferred-throat-tropic isolates no preferential adherence was observed to a particular cell line. The 23/69 strains classified as 'generalists', however, showed an overall greater ability to adhere to both cell lines. CONCLUSIONS: Predicted tissue-tropism based on emm patterns is not reflected by preferential adherence to a specific cell line, suggesting that early adhesion events may not be as important in establishing infection at a particular ecological niche than originally expected.
Authors: Patricia Therese Campbell; Steven Y C Tong; Nicholas Geard; Mark R Davies; Kate A Worthing; Jake A Lacey; Pierre R Smeesters; Michael R Batzloff; Joseph Kado; Adam W J Jenney; Jodie Mcvernon; Andrew C Steer Journal: J Infect Dis Date: 2020-04-07 Impact factor: 7.759
Authors: Joshua Osowicki; Kristy I Azzopardi; Liam McIntyre; Tania Rivera-Hernandez; Cheryl-Lynn Y Ong; Ciara Baker; Christine M Gillen; Mark J Walker; Pierre R Smeesters; Mark R Davies; Andrew C Steer Journal: mSphere Date: 2019-02-13 Impact factor: 4.389