Literature DB >> 2833296

Transforming growth factor-beta reduces the phenotypic expression of osteoblastic MC3T3-E1 cells in monolayer culture.

P R Elford1, H L Guenther, R Felix, M G Cecchini, H Fleisch.   

Abstract

Transforming growth factor beta (TGF-beta) regulates cell growth and differentiation. Since it is abundant in bone, we have studied the effect of the polypeptide upon the growth and phenotypic expression of murine osteoblastic cells in monolayer culture. Its actions were compared to those of epidermal growth factor (EGF), another hormonally active polypeptide known to alter bone cell function. Picogram amounts of TGF-beta were found to inhibit the growth and phenotype (alkaline phosphatase and cAMP response to parathyroid hormone) of the clonal nontransformed MC3T3-E1 osteoblastic cell line. EGF also inhibited phenotypic expression, although at higher (nanogram) concentrations, but stimulated cell growth. The low concentration of TGF-beta required to inhibit growth and phenotype of osteoblastic cells together with its abundance in bone suggest that TGF-beta may be an important regulator of bone cell function.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 2833296     DOI： 10.1016/8756-3282(87)90174-8

Source DB:  PubMed          Journal:  Bone        ISSN： 1873-2763            Impact factor:   4.398


  11 in total

1.  Effects of transforming growth factor-beta1 on the gene expression of decorin, biglycan, and alkaline phosphatase in osteoblast precursor cells and more differentiated osteoblast cells.

Authors:  T Yamada; N Kamiya; D Harada; M Takagi
Journal:  Histochem J       Date:  1999-10

2.  Growth factors regulate expression of mineral associated genes in cementoblasts.

Authors:  N E Saygin; Y Tokiyasu; W V Giannobile; M J Somerman
Journal:  J Periodontol       Date:  2000-10       Impact factor: 6.993

3.  Transforming growth factor-β receptors.

Authors:  P R Segarini
Journal:  Cytotechnology       Date:  1989-12       Impact factor: 2.058

Review 4.  Hypercalcaemia of malignancy.

Authors:  P J Kelly; J A Eisman
Journal:  Cancer Metastasis Rev       Date:  1989-06       Impact factor: 9.264

5.  The effects of the insulin-like growth factors and transforming growth factor beta on the Jun proto-oncogene family in MC3T3-E1 cells.

Authors:  D D Strong; H L Merriman; E C Landale; D J Baylink; S Mohan
Journal:  Calcif Tissue Int       Date:  1994-10       Impact factor: 4.333

6.  Bone-resorbing cytokines enhance release of macrophage colony-stimulating activity by the osteoblastic cell MC3T3-E1.

Authors:  R Felix; H Fleisch; P R Elford
Journal:  Calcif Tissue Int       Date:  1989-05       Impact factor: 4.333

7.  Transforming growth factor-beta inhibition of mineralization by neonatal rat osteoblasts in monolayer and collagen gel culture.

Authors:  D J Talley-Ronsholdt; E Lajiness; K Nagodawithana
Journal:  In Vitro Cell Dev Biol Anim       Date:  1995-04       Impact factor: 2.416

8.  Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling.

Authors:  Jila Nasirzade; Zahra Kargarpour; Goran Mitulović; Franz Josef Strauss; Layla Panahipour; Frank Schwarz; Reinhard Gruber
Journal:  Sci Rep       Date:  2021-06-10       Impact factor: 4.996

9.  Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro.

Authors:  A Yamaguchi; T Katagiri; T Ikeda; J M Wozney; V Rosen; E A Wang; A J Kahn; T Suda; S Yoshiki
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539

10.  Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage.

Authors:  T Katagiri; A Yamaguchi; M Komaki; E Abe; N Takahashi; T Ikeda; V Rosen; J M Wozney; A Fujisawa-Sehara; T Suda
Journal:  J Cell Biol       Date:  1994-12       Impact factor: 10.539
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.