Literature DB >> 2833127

A tissue culture system for the growth of several species of Leishmania: growth kinetics and drug sensitivities.

S Martinez1, D L Looker, J J Marr.   

Abstract

We have developed a simple in vitro method of infecting a continuous human macrophage cell line (U937) with promastigotes of several species of Leishmania. These include L. braziliensis braziliensis, L. b. panamensis, L. donovani, L. mexicana mexicana, L. m. pifanoi, L. tropica, and L. major. The growth kinetics of these species are presented as well as drug sensitivity data. The U937 cell system can be used to determine drug efficacy and eliminates the need to use amastigotes from animal tissues to infect the tissue culture.

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Year:  1988        PMID： 2833127     DOI： 10.4269/ajtmh.1988.38.304

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  5 in total

Review 1.  Clinical and experimental advances in treatment of visceral leishmaniasis.

Authors:  H W Murray
Journal:  Antimicrob Agents Chemother       Date:  2001-08       Impact factor: 5.191

2.  Use of in vitro method to assess different brands of anti-leishmanial drugs.

Authors:  S K Arora; R Sinha; S Sehgal
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402

3.  Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients.

Authors:  J A Kurtzhals; A S Hey; A Jardim; M Kemp; K U Schaefer; E O Odera; C B Christensen; J I Githure; R W Olafson; T G Theander
Journal:  Clin Exp Immunol       Date:  1994-06       Impact factor: 4.330

4.  Dichotomy of the T cell response to Leishmania antigens in patients suffering from cutaneous leishmaniasis; absence or scarcity of Th1 activity is associated with severe infections.

Authors:  A Gaafar; A Kharazmi; A Ismail; M Kemp; A Hey; C B Christensen; M Dafalla; A Y el Kadaro; A M el Hassan; T G Theander
Journal:  Clin Exp Immunol       Date:  1995-05       Impact factor: 4.330

5.  Axenically grown amastigotes of Leishmania infantum used as an in vitro model to investigate the pentavalent antimony mode of action.

Authors:  D Sereno; M Cavaleyra; K Zemzoumi; S Maquaire; A Ouaissi; J L Lemesre
Journal:  Antimicrob Agents Chemother       Date:  1998-12       Impact factor: 5.191
  5 in total

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