Wanling Yao1, Chaoxue Yang2, Yanqiao Wen3, Wangdong Zhang4, Xiaosong Zhang5, Qi Ma6, Peng Ji7, Yongli Hua8, Yanming Wei9. 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: kololo-2008@163.com. 2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 1720601837@qq.com. 3. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 13659465092@163.com. 4. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: awang001@126.com. 5. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: 770455708@qq.com. 6. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: mq652@qq.com. 7. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: jipengjipeng528@163.com. 8. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: huayongli2004@163.com. 9. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China. Electronic address: weiym@gsau.edu.cn.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Yujin Powder (YJP), an old prescription, is one of the most classical prescription for treating the large intestine dampness-heat syndrome (LIDHS). However, its potential modern pharmacological mechanisms remain unclear. AIM OF THE STUDY: The present study was designed to explore the essence of LIDHS and treatment mechanisms of the YJP on the LIDHS. METHODS: The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment (HTHE), drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of LIDHS. Then the clinical symptoms and signs, blood routine, blood biochemistry, whole blood viscosity (WBV), serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed, respectively. RESULTS: The results showed that the clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of LIDHS, moreover, there were obvious systemic inflammatory response and extensive congestion. And after treatment with YJP in different dosages, the clinical symptoms and signs of the rats with LIDHS were improved; the indexes of blood routine and blood biochemistry and inflammatory cytokines levels tended to be normal; the WBV decreased and histopathological changes of major organs were alleviated or returned to normal. There was an obvious dose-effect relationship, and the high dose of YJP (HD-YJP) had the best treatment effects. CONCLUSIONS: These results suggested that in LIDHS, diarrhea was the major clinical manifestation; the large intestine was the main lesion area; mucosa injury, inflammation and congestion of the large intestine with systemic inflammatory response and congestion were the most typical pathological characteristics. Meanwhile, YJP exhibited the comprehensive effects of anti-diarrhea, anti-inflammation, lowering blood lipid, relieving blood stasis, repairing intestinal mucosa and regulation and protection of multiple organs on LIDHS. These findings provided not only important information for understanding the essence of LIDHS but also the theoretical basis for developing new-drugs for treating dampness-heat type of diarrheal diseases.
ETHNOPHARMACOLOGICAL RELEVANCE: Yujin Powder (YJP), an old prescription, is one of the most classical prescription for treating the large intestine dampness-heat syndrome (LIDHS). However, its potential modern pharmacological mechanisms remain unclear. AIM OF THE STUDY: The present study was designed to explore the essence of LIDHS and treatment mechanisms of the YJP on the LIDHS. METHODS: The rat model of LIDHS was established by such complex factors as high-sugar and high-fat diet, improper diet, high temperature and humidity environment (HTHE), drinking and intraperitoneal injection of Escherichia coli., which imitated the inducing conditions of LIDHS. Then the clinical symptoms and signs, blood routine, blood biochemistry, whole blood viscosity (WBV), serum inflammatory cytokines levels and the histopathological changes of main organs were detected and observed, respectively. RESULTS: The results showed that the clinical symptoms and signs of the model rats were consistent with the diagnostic criteria of LIDHS, moreover, there were obvious systemic inflammatory response and extensive congestion. And after treatment with YJP in different dosages, the clinical symptoms and signs of the rats with LIDHS were improved; the indexes of blood routine and blood biochemistry and inflammatory cytokines levels tended to be normal; the WBV decreased and histopathological changes of major organs were alleviated or returned to normal. There was an obvious dose-effect relationship, and the high dose of YJP (HD-YJP) had the best treatment effects. CONCLUSIONS: These results suggested that in LIDHS, diarrhea was the major clinical manifestation; the large intestine was the main lesion area; mucosa injury, inflammation and congestion of the large intestine with systemic inflammatory response and congestion were the most typical pathological characteristics. Meanwhile, YJP exhibited the comprehensive effects of anti-diarrhea, anti-inflammation, lowering blood lipid, relieving blood stasis, repairing intestinal mucosa and regulation and protection of multiple organs on LIDHS. These findings provided not only important information for understanding the essence of LIDHS but also the theoretical basis for developing new-drugs for treating dampness-heat type of diarrheal diseases.
Authors: Zunxiang Yan; Kang Zhang; Kai Zhang; Guibo Wang; Lei Wang; Jingyan Zhang; Zhengying Qiu; Zhiting Guo; Xiaoping Song; Jianxi Li Journal: Front Vet Sci Date: 2022-02-15
Authors: Zunxiang Yan; Kang Zhang; Guibo Wang; Lei Wang; Jingyan Zhang; Zhengying Qiu; Zhiting Guo; Kai Zhang; Jianxi Li Journal: Front Vet Sci Date: 2022-09-20