Literature DB >> 28330214

Production kinetics of polyhydroxyalkanoates by using Pseudomonas aeruginosa gamma ray mutant strain EBN-8 cultured on soybean oil.

Sharjeel Abid1, Zulfiqar Ali Raza2, Tanveer Hussain1.   

Abstract

The purpose of present study was to optimize polyhydroxyalkanotes (PHAs) production in a gamma ray mutant strain of Pseudomonas aeruginosa grown on soybean oil in minimal salts media under shake flask conditions. The production kinetics was studied by sampling on daily basis for 6 days to investigate the best conditions for PHAs production like biomass estimation, carbon source utilization and PHAs yield. The PHA accumulation was observed up to 50.27 % (w/w) of cell dry mass. The Pseudomonas species synthesized medium chain length PHA copolyester as per identified by LCMS and confirmed by FTIR spectroscopy. The ESI-MS analysis exhibited the major polyhydroxybutyrate with a molecular mass of m/z 448.5.

Entities:  

Keywords:  PHAs; Polyhydroxyalkanoates; Pseudomonas aeruginosa; Shake flask; Soybean oil

Year:  2016        PMID： 28330214      PMCID： PMC4919136          DOI： 10.1007/s13205-016-0452-4

Source DB:  PubMed          Journal:  3 Biotech        ISSN： 2190-5738            Impact factor:   2.406


  25 in total

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9.  Pseudomonas extremaustralis sp. nov., a Poly(3-hydroxybutyrate) producer isolated from an antarctic environment.

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