Christian Castillo1, Lorena Muñoz1, Ileana Carrillo1, Ana Liempi1, Christian Gallardo1, Norbel Galanti2, Juan Diego Maya3, Ulrike Kemmerling1. 1. Programa de Anatomía y Biología del Desarrollo, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile. 2. Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile. 3. Programa de Farmacología Molecular y Clínica, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Abstract
PROBLEM: Trypanosoma cruzi and Toxoplasma gondii present, respectively, low and high congenital transmission rates. The placenta as an immune regulatory organ expresses TLRs, leading to the secretion of cytokines. Both parasites are recognized by TLR-2, TLR-4, and TLR-9. Here, we studied if the parasites induce differences in TLR protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection. METHOD OF STUDY: Placental tissue explants were infected ex vivo with each parasite, TLRs protein expression, cytokine profile and parasite infection were determined by Western blotting, ELISA and qPCR. RESULTS: Trypanosoma cruzi and Toxoplasma gondii infection is related to TLR-2 and TLR-4/TLR-9, respectively. Trypanosoma cruzi elicits an increase in TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine secretion whereas T. gondii only increases the secretion of IL-8. CONCLUSION: The susceptibility of the placenta to each parasite is mediated partially by the innate immune response.
PROBLEM: Trypanosoma cruzi and Toxoplasma gondii present, respectively, low and high congenital transmission rates. The placenta as an immune regulatory organ expresses TLRs, leading to the secretion of cytokines. Both parasites are recognized by TLR-2, TLR-4, and TLR-9. Here, we studied if the parasites induce differences in TLR protein expression, cytokine profiles, and whether receptor inhibition is related to parasite infection. METHOD OF STUDY: Placental tissue explants were infected ex vivo with each parasite, TLRs protein expression, cytokine profile and parasite infection were determined by Western blotting, ELISA and qPCR. RESULTS:Trypanosoma cruzi and Toxoplasma gondii infection is related to TLR-2 and TLR-4/TLR-9, respectively. Trypanosoma cruzi elicits an increase in TNF-α, IL-1β, IL-6, IL-8 and IL-10 cytokine secretion whereas T. gondii only increases the secretion of IL-8. CONCLUSION: The susceptibility of the placenta to each parasite is mediated partially by the innate immune response.
Authors: Maura Rojas-Pirela; Diego Andrade-Alviárez; Lisvaneth Medina; Christian Castillo; Ana Liempi; Jesús Guerrero-Muñoz; Yessica Ortega; Juan Diego Maya; Verónica Rojas; Wilfredo Quiñones; Paul A Michels; Ulrike Kemmerling Journal: Open Biol Date: 2022-06-15 Impact factor: 7.124
Authors: Iván Pastor-Fernández; Esther Collantes-Fernández; Laura Jiménez-Pelayo; Luis Miguel Ortega-Mora; Pilar Horcajo Journal: Front Vet Sci Date: 2021-01-21
Authors: Fernando Gómez-Chávez; Irma Cañedo-Solares; Luz Belinda Ortiz-Alegría; Yevel Flores-García; Ricardo Figueroa-Damián; Héctor Luna-Pastén; Valeria Gómez-Toscano; Carlos López-Candiani; G Emmanuel Arce-Estrada; Christian A Bonilla-Ríos; Juan Carlos Mora-González; Ricardo García-Ruiz; Dolores Correa Journal: Front Immunol Date: 2020-03-13 Impact factor: 7.561