Min He1,2, Amy C Harms1, Eduard van Wijk1,2, Mei Wang1,2,3, Ruud Berger1, Slavik Koval1, Thomas Hankemeier1,2, Jan van der Greef1,2,4. 1. Analytical BioSciences, LACDR, Leiden University, Leiden, the Netherlands. 2. Sino-Dutch Center for Preventive and Personalized Medicine, Leiden University, Leiden, the Netherlands. 3. SU Biomedicine, Zeist, the Netherlands. 4. TNO, Zeist, the Netherlands.
Abstract
BACKGROUND: Rheumatoid arthritis (RA) is a complex, chronic autoimmune disease characterized by various inflammatory symptoms, including joint swelling, joint pain, and both structural and functional joint damage. The most commonly used animal model for studying RA is mice with collagen-induced arthritis (CIA); the wide use of this model is due primarily to many similarities with RA in human patients. Metabolomics is used increasingly in biological studies for diagnosing disease and for predicting and evaluating drug interventions, as a large number of disease-associated metabolites can be analyzed and interpreted from a biological perspective. AIM: To profile free amino acids and their biogenic metabolites in CIA mice plasma. METHOD: Ultra-high-performance liquid chromatography/tandem mass spectrometry coupled with multiple reaction monitoring (MRM) was used for metabolomics study. RESULTS: Profile of 45 amine metabolites, including free amino acids and their biogenic metabolites in plasma was obtained from CIA mice. We found that the plasma levels of 20 amine metabolites were significantly decreased in the CIA group. CONCLUSION: The results suggest that a disordered amine response is linked to RA-associated muscle wasting and energy expenditure.
BACKGROUND:Rheumatoid arthritis (RA) is a complex, chronic autoimmune disease characterized by various inflammatory symptoms, including joint swelling, joint pain, and both structural and functional joint damage. The most commonly used animal model for studying RA is mice with collagen-induced arthritis (CIA); the wide use of this model is due primarily to many similarities with RA in humanpatients. Metabolomics is used increasingly in biological studies for diagnosing disease and for predicting and evaluating drug interventions, as a large number of disease-associated metabolites can be analyzed and interpreted from a biological perspective. AIM: To profile free amino acids and their biogenic metabolites in CIA mice plasma. METHOD: Ultra-high-performance liquid chromatography/tandem mass spectrometry coupled with multiple reaction monitoring (MRM) was used for metabolomics study. RESULTS: Profile of 45 amine metabolites, including free amino acids and their biogenic metabolites in plasma was obtained from CIA mice. We found that the plasma levels of 20 amine metabolites were significantly decreased in the CIA group. CONCLUSION: The results suggest that a disorderedamine response is linked to RA-associated muscle wasting and energy expenditure.
Authors: John M Davis; Jaeyun Sung; Benjamin Hur; Vinod K Gupta; Harvey Huang; Kerry A Wright; Kenneth J Warrington; Veena Taneja Journal: Arthritis Res Ther Date: 2021-06-08 Impact factor: 5.156
Authors: Yi Ding; Robert-Jan Raterink; Rubén Marín-Juez; Wouter J Veneman; Koen Egbers; Susan van den Eeden; Mariëlle C Haks; Simone A Joosten; Tom H M Ottenhoff; Amy C Harms; A Alia; Thomas Hankemeier; Herman P Spaink Journal: Sci Rep Date: 2020-07-15 Impact factor: 4.379