| Literature DB >> 2832478 |
Abstract
A rapid and efficient procedure for the isolation of Epstein-Barr (EB) virus membrane glycoprotein gp340 has been developed. The mild, two step method consisted of anion exchange chromatography of B95-8 cell membrane detergent extracts followed by further purification by gel filtration. Production of homogeneous gp340 was achieved routinely with recoveries of at least 35% from starting material. The purified molecule, when injected into mice with a synthetic muramyl dipeptide adjuvant, N-acetylmuramyl-L-threonyl-D-isoglutamine ((Thr1)MDP), elicited high titres of EB virus-neutralizing antibody.Entities:
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Year: 1988 PMID： 2832478 DOI： 10.1016/0022-1759(88)90424-3
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303