| Literature DB >> 28324602 |
Peter Quicke1,2,3, Samuel J Barnes3, Thomas Knöpfel4,5.
Abstract
Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.Entities:
Keywords: Brain slices; Fluorescence imaging; Fluorescence microscopy; Voltage imaging; Voltage indicators; Voltage sensitive fluorescent proteins
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Year: 2017 PMID： 28324602 PMCID： PMC5939932 DOI： 10.1007/978-1-4939-6810-7_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745