Literature DB >> 28324495

Telomere Length Analysis by Quantitative Fluorescent in Situ Hybridization (Q-FISH).

Isabelle Ourliac-Garnier1,2, Arturo Londoño-Vallejo3,4.   

Abstract

Length is a functional parameter of telomeres, the nucleoprotein structures that protect chromosome ends. The availability of highly specific, high affinity probes for telomeric repeat sequences allowed the development of quantitative approaches aimed at measuring telomere length directly on chromosomes or in interphase nuclei. Here, we describe a general method for telomere quantitative FISH on metaphase chromosomes and discuss its most common applications in research.

Keywords:  FISH; LNA; Length; PNA; Q-FISH; Telomere

Mesh：

Year:  2017        PMID： 28324495     DOI： 10.1007/978-1-4939-6892-3_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  From incomplete penetrance with normal telomere length to severe disease and telomere shortening in a family with monoallelic and biallelic PARN pathogenic variants.

Authors:  Lois M Dodson; Alessandro Baldan; Mikael Nissbeck; Sethu M R Gunja; Penelope E Bonnen; Geraldine Aubert; Sherri Birchansky; Anders Virtanen; Alison A Bertuch
Journal:  Hum Mutat       Date:  2019-09-15       Impact factor: 4.878

2.  Telomerase therapy reverses vascular senescence and extends lifespan in progeria mice.

Authors:  Anahita Mojiri; Brandon K Walther; Chongming Jiang; Gianfranco Matrone; Rhonda Holgate; Qiu Xu; Elisa Morales; Guangyu Wang; Jianhua Gu; Rongfu Wang; John P Cooke
Journal:  Eur Heart J       Date:  2021-11-07       Impact factor: 35.855

3.  RAD52 and SLX4 act nonepistatically to ensure telomere stability during alternative telomere lengthening.

Authors:  Robert L Dilley; Tianpeng Zhang; Priyanka Verma; Melina T Gyparaki; Yiwen Li; Roger A Greenberg
Journal:  Genes Dev       Date:  2019-01-28       Impact factor: 11.361

4.  Biallelic variants in YRDC cause a developmental disorder with progeroid features.

Authors:  Julia Schmidt; Jonas Goergens; Tatiana Pochechueva; Annika Kotter; Niko Schwenzer; Maren Sitte; Gesa Werner; Janine Altmüller; Holger Thiele; Peter Nürnberg; Jörg Isensee; Yun Li; Christian Müller; Barbara Leube; H Christian Reinhardt; Tim Hucho; Gabriela Salinas; Mark Helm; Ron D Jachimowicz; Dagmar Wieczorek; Tobias Kohl; Stephan E Lehnart; Gökhan Yigit; Bernd Wollnik
Journal:  Hum Genet       Date:  2021-09-20       Impact factor: 4.132

5.  Analytical Validation of Telomere Analysis Technology® for the High-Throughput Analysis of Multiple Telomere-Associated Variables.

Authors:  Nuria de Pedro; María Díez; Irene García; Jorge García; Lissette Otero; Luis Fernández; Beatriz García; Rut González; Sara Rincón; Diego Pérez; Estefanía Rodríguez; Enrique Segovia; Pilar Najarro
Journal:  Biol Proced Online       Date:  2020-01-15       Impact factor: 3.244
  5 in total

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