Pallavi Thakur1, Raman Chawla2, Alka Narula3, Rakesh Kumar Sharma1. 1. Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, New Delhi 110054, India. 2. Division of CBRN Defence, Institute of Nuclear Medicine and Allied Sciences, New Delhi 110054, India. Electronic address: ptrcmanuscripts@gmail.com. 3. Department of Biotechnology, Jamia Hamdard, New Delhi, India.
Abstract
OBJECTIVES: Berberis aristata is known to contain a variety of phenolic compounds contributing to its holistic capability of mitigating bacterial multidrug resistance. METHODS: B. aristata stem bark extract was prepared and was characterised using liquid chromatography-mass spectrometry (LC-MS). The antimicrobial efficacy of the extract against carbapenem-resistant Escherichia coli was assessed in vivo in an animal model using Sprague Dawley rats. Microbial counts in blood and urine, physical health status, haematological and biochemical analysis of blood, and histopathology of the kidney were assessed as the study endpoints. RESULTS: An aquo-alcoholic extract of B. aristata (PTRC-2111-A) was found to effectively manage peritonitis induced by carbapenem-resistant E. coli in a rat model at a single post-exposure prophylactic dose of 0.5mg/kg body weight (BW). The extract was also found to show a no observed adverse effect level (NOAEL) up to a dose of 2000mg/kg BW. Physical, immunological, haematological, biochemical and histopathological aberrations were found to be restored to normal in the herbal-treated group at a dose of 0.5mg/kg BW. CONCLUSIONS: The antimicrobial and hepatorenal protective ability of PTRC-2111-A could be attributed to the presence of isoquinoline alkaloids.
OBJECTIVES:Berberis aristata is known to contain a variety of phenolic compounds contributing to its holistic capability of mitigating bacterial multidrug resistance. METHODS:B. aristata stem bark extract was prepared and was characterised using liquid chromatography-mass spectrometry (LC-MS). The antimicrobial efficacy of the extract against carbapenem-resistant Escherichia coli was assessed in vivo in an animal model using Sprague Dawley rats. Microbial counts in blood and urine, physical health status, haematological and biochemical analysis of blood, and histopathology of the kidney were assessed as the study endpoints. RESULTS: An aquo-alcoholic extract of B. aristata (PTRC-2111-A) was found to effectively manage peritonitis induced by carbapenem-resistant E. coli in a rat model at a single post-exposure prophylactic dose of 0.5mg/kg body weight (BW). The extract was also found to show a no observed adverse effect level (NOAEL) up to a dose of 2000mg/kg BW. Physical, immunological, haematological, biochemical and histopathological aberrations were found to be restored to normal in the herbal-treated group at a dose of 0.5mg/kg BW. CONCLUSIONS: The antimicrobial and hepatorenal protective ability of PTRC-2111-A could be attributed to the presence of isoquinoline alkaloids.
Authors: Bahare Salehi; Zeliha Selamoglu; Bilge Sener; Mehtap Kilic; Arun Kumar Jugran; Nunziatina de Tommasi; Chiara Sinisgalli; Luigi Milella; Jovana Rajkovic; Maria Flaviana B Morais-Braga; Camila F Bezerra; Janaína E Rocha; Henrique D M Coutinho; Adedayo Oluwaseun Ademiluyi; Zabta Khan Shinwari; Sohail Ahmad Jan; Ebru Erol; Zulfiqar Ali; Elise Adrian Ostrander; Javad Sharifi-Rad; María de la Luz Cádiz-Gurrea; Yasaman Taheri; Miquel Martorell; Antonio Segura-Carretero; William C Cho Journal: Foods Date: 2019-10-22