AIM: To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death. METHODS: Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied. RESULTS: ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from H2O2-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death. CONCLUSION: ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death.
AIM: To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death. METHODS: Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied. RESULTS:ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from H2O2-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death. CONCLUSION:ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death.
Authors: Aaser M Abdelazim; Islam M Saadeldin; Ayman Abdel-Aziz Swelum; Mohamed M Afifi; Ali Alkaladi Journal: Oxid Med Cell Longev Date: 2018-04-05 Impact factor: 6.543