Literature DB >> 2832251

Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus.

O Chisaka1, K Araki, T Ochiya, T Tsurimoto, W Hiranyawasitte-Attatippaholkun, N Yanaihara, K Matsubara.   

Abstract

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

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Year:  1987        PMID： 2832251     DOI： 10.1016/0378-1119(87)90226-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  9 in total

1.  XAP2, a novel hepatitis B virus X-associated protein that inhibits X transactivation.

Authors:  N Kuzhandaivelu; Y S Cong; C Inouye; W M Yang; E Seto
Journal:  Nucleic Acids Res       Date:  1996-12-01       Impact factor: 16.971

2.  Transcriptional activation of homologous and heterologous genes by the hepatitis B virus X gene product in cells permissive for viral replication.

Authors:  R Colgrove; G Simon; D Ganem
Journal:  J Virol       Date:  1989-09       Impact factor: 5.103

3.  Expression of the X protein of hepatitis B virus in insect cells using recombinant baculoviruses.

Authors:  R Klein; C H Schröder; H Zentgraf
Journal:  Virus Genes       Date:  1991-04       Impact factor: 2.332

4.  Hepatitis B virus transactivator X protein is not tumorigenic in transgenic mice.

Authors:  T H Lee; M J Finegold; R F Shen; J L DeMayo; S L Woo; J S Butel
Journal:  J Virol       Date:  1990-12       Impact factor: 5.103

5.  Mapping of B-cell epitopes of the human hepatitis B virus X protein.

Authors:  M Stemler; T Weimer; Z X Tu; D F Wan; M Levrero; C Jung; G R Pape; H Will
Journal:  J Virol       Date:  1990-06       Impact factor: 5.103

6.  Activation of class I major histocompatibility complex gene expression by hepatitis B virus.

Authors:  D X Zhou; A Taraboulos; J H Ou; T S Yen
Journal:  J Virol       Date:  1990-08       Impact factor: 5.103

7.  Hepatitis B virus X protein activates transcription factor NF-kappa B without a requirement for protein kinase C.

Authors:  R Lucito; R J Schneider
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

8.  The hepatitis B virus X-gene product trans-activates both RNA polymerase II and III promoters.

Authors:  B Aufiero; R J Schneider
Journal:  EMBO J       Date:  1990-02       Impact factor: 11.598

9.  The hepatitis B virus HBx protein is a dual specificity cytoplasmic activator of Ras and nuclear activator of transcription factors.

Authors:  M Doria; N Klein; R Lucito; R J Schneider
Journal:  EMBO J       Date:  1995-10-02       Impact factor: 11.598
  9 in total

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