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Literature DB >> 2832136

In situ nick translation distinguishes between C-band positive regions on mouse chromosomes.

Abstract

In situ nick translation of mouse metaphase chromosomes by non-radioactive detection means and DNase I digestion followed by Giemsa staining were used to analyse the DNase I resistance of two different C-band positive regions. These were the centromeric heterochromatin of acro- and metacentric chromosomes and an interstitial C-band on chromosome 1 of wild mice, IS(HSR;1C5D)1Lub. Whereas the centromeric heterochromatin was clearly resistant to DNase I, the interstitial C-band showed very high DNase I sensitivity. Among centromeric C-bands, the heterochromatin in Robertsonian fusion biarmed chromosomes was more resistant to DNase I action than was the centromeric heterochromatin of the acrocentric chromosomes.

Entities: Gene Species

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Substances：

Year: 1988 PMID： 2832136 DOI： 10.1007/BF00331041

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

20 in total

1. Highly regular arrangement of a restriction-nuclease-sensitive site in rodent satellite DNAs.

Authors: W Hörz; I Hess; H G Zachau
Journal: Eur J Biochem Date: 1974-06-15

2. Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization.

Authors: F E Arrighi; T C Hsu; P Saunders; G F Saunders
Journal: Chromosoma Date: 1970 Impact factor: 4.316

Review 3. DNAase I-hypersensitive sites of chromatin.

Authors: S C Elgin
Journal: Cell Date: 1981-12 Impact factor: 41.582

4. Restriction enzyme banding of mouse metaphase chromosomes.

Authors: M Kaelbling; D A Miller; O J Miller
Journal: Chromosoma Date: 1984 Impact factor: 4.316

5. Mapping of DNAase I sensitive regions on mitotic chromosomes.

Authors: B S Kerem; R Goitein; G Diamond; H Cedar; M Marcus
Journal: Cell Date: 1984-09 Impact factor: 41.582

Review 6. Active chromatin.

Authors: S Weisbrod
Journal: Nature Date: 1982-05-27 Impact factor: 49.962

7. Active genes are sensitive to deoxyribonuclease I during metaphase.

Authors: B Gazit; H Cedar; I Lerer; R Voss
Journal: Science Date: 1982-08-13 Impact factor: 47.728

8. Anatomy of hypersensitive sites.

Authors: S C Elgin
Journal: Nature Date: 1984 May 17-23 Impact factor: 49.962

9. In situ nick translation of metaphase chromosomes with biotin-labeled d-UTP.

Authors: S Adolph; H Hameister
Journal: Hum Genet Date: 1985 Impact factor: 4.132

10. A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.

Authors: M Schwab; K Alitalo; H E Varmus; J M Bishop; D George
Journal: Nature Date: 1983 Jun 9-15 Impact factor: 49.962

11 in total

1. Restriction endonuclease/nick translation of fixed mouse chromosomes: a study of factors affecting digestion of chromosomal DNA in situ.

Authors: J de la Torre; A R Mitchell; A T Summer
Journal: Chromosoma Date: 1991-03 Impact factor: 4.316

9. Nonradioactive in situ nick translation combined with counterstaining: characterization of C-band and silver positive regions in mouse testicular cells.

Authors: S Adolph; C Klett; A Weith
Journal: Chromosoma Date: 1990-08 Impact factor: 4.316

10. Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.

Authors: D A Loebel; P G Johnston
Journal: Chromosoma Date: 1993-01 Impact factor: 4.316

In situ nick translation distinguishes between C-band positive regions on mouse chromosomes.

1. Highly regular arrangement of a restriction-nuclease-sensitive site in rodent satellite DNAs.

2. Localization of repetitive DNA in the chromosomes of Microtus agrestis by means of in situ hybridization.

Review 3. DNAase I-hypersensitive sites of chromatin.

4. Restriction enzyme banding of mouse metaphase chromosomes.

5. Mapping of DNAase I sensitive regions on mitotic chromosomes.

Review 6. Active chromatin.

7. Active genes are sensitive to deoxyribonuclease I during metaphase.

8. Anatomy of hypersensitive sites.

9. In situ nick translation of metaphase chromosomes with biotin-labeled d-UTP.

10. A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.

1. Restriction endonuclease/nick translation of fixed mouse chromosomes: a study of factors affecting digestion of chromosomal DNA in situ.

Review 2. Restriction endonucleases in the study of eukaryotic chromosomes.

3. Patterns of digestion of human chromosomes by restriction endonucleases demonstrated by in situ nick translation.

4. Patterns of DNase I sensitivity in the chromosomes of the grasshopper Chorthippus parallelus (Orthoptera).

5. Morphological and biochemical effects of endonucleases on isolated mammalian chromosomes in vitro.

6. Restriction endonuclease/nick translation procedure on fixed chromosomes of the Atlantic salmon fish cell line.

7. Digestion of centromeric DNA from each human metaphase chromosome by the 6 bp-restriction enzyme StuI.

8. Analysis of chromosome replication by a BrdU antibody technique.

9. Nonradioactive in situ nick translation combined with counterstaining: characterization of C-band and silver positive regions in mouse testicular cells.

10. Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.