Takashi Miida1, Kunihiro Nishimura2, Satoshi Hirayama1, Yoshihiro Miyamoto3, Masakazu Nakamura4, Daisaku Masuda5, Shizuya Yamashita5,6,7, Masaji Ushiyama8, Toshiaki Komori8, Naohisa Fujita9, Shinji Yokoyama10, Tamio Teramoto11. 1. Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine. 2. Department of Preventive Medicine and Epidemiologic Informatics, National Cerebral and Cardiovascular Center. 3. Department of Preventive Cardiology, Department of Preventive Medicine and Epidemiologic Informatics, National Cerebral and Cardiovascular Center. 4. Department of Preventive Cardiology, Lipid Reference Laboratory, National Cerebral and Cardiovascular Center. 5. Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine. 6. Rinku General Medical Center. 7. Department of Community Medicine, Osaka University Graduate School of Medicine. 8. Clinical Laboratory, University Hospital, Kyoto Prefectural University of Medicine. 9. Department of Infection Control and Laboratory Medicine, Kyoto Prefectural University of Medicine. 10. Department of Food and Nutritional Sciences, Practice Center for Registered Dietitian, Chubu University. 11. Teikyo Academic Research Center, Teikyo University.
Abstract
AIM: Most epidemiological and clinical studies calculated low-density lipoprotein-cholesterol (LDL-C) by Friedewald's formula which cannot be used in the postprandial samples. Although the homogeneous assays with poor analytical performance were withdrawn from the market, it remained unclear whether the currently available reagents for LDL-C and high-density lipoprotein-cholesterol (HDL-C) are as accurate for postprandial samples as for fasting samples. METHODS: Fresh blood samples were collected from 59 non-diseased and 109 diseased subjects. Postprandial samples constituted 72.9% and 39.4% of these samples. LDL-C and HDL-C concentrations were measured using the homogeneous assays of four manufacturers (Denka Seiken, Wako, Kyowa Medex, and Sekisui Medical). Simultaneously, LDL-C and HDL-C concentrations were determined using the reference measurement procedures (RMPs) of the Centers for Disease Control and Prevention (CDC). Total errors were calculated using a routine method (TEcom) and via error component analysis (TEECA). RESULTS: All homogeneous assays for LDL-C and HDL-C met the National Cholesterol Education Program (NCEP) requirements in terms of coefficient of variation, and TEcom in both non-diseased and diseased subjects. LDL-C and HDL-C values measured by the homogeneous assays were in good agreement with those measured by the RMPs in both fasting and postprandial samples. The TEcom and TEECA values of the postprandial samples were similar to those of fasting samples, although the TEECA values were up to 4.4-fold greater than the TEcom values. CONCLUSIONS: In both non-diseased and diseased subjects, the homogeneous assays for LDL-C and HDL-C of four manufacturers are as accurate for postprandial samples as for fasting samples.
AIM: Most epidemiological and clinical studies calculated low-density lipoprotein-cholesterol (LDL-C) by Friedewald's formula which cannot be used in the postprandial samples. Although the homogeneous assays with poor analytical performance were withdrawn from the market, it remained unclear whether the currently available reagents for LDL-C and high-density lipoprotein-cholesterol (HDL-C) are as accurate for postprandial samples as for fasting samples. METHODS: Fresh blood samples were collected from 59 non-diseased and 109 diseased subjects. Postprandial samples constituted 72.9% and 39.4% of these samples. LDL-C and HDL-C concentrations were measured using the homogeneous assays of four manufacturers (Denka Seiken, Wako, Kyowa Medex, and Sekisui Medical). Simultaneously, LDL-C and HDL-C concentrations were determined using the reference measurement procedures (RMPs) of the Centers for Disease Control and Prevention (CDC). Total errors were calculated using a routine method (TEcom) and via error component analysis (TEECA). RESULTS: All homogeneous assays for LDL-C and HDL-C met the National Cholesterol Education Program (NCEP) requirements in terms of coefficient of variation, and TEcom in both non-diseased and diseased subjects. LDL-C and HDL-C values measured by the homogeneous assays were in good agreement with those measured by the RMPs in both fasting and postprandial samples. The TEcom and TEECA values of the postprandial samples were similar to those of fasting samples, although the TEECA values were up to 4.4-fold greater than the TEcom values. CONCLUSIONS: In both non-diseased and diseased subjects, the homogeneous assays for LDL-C and HDL-C of four manufacturers are as accurate for postprandial samples as for fasting samples.
Authors: H Iso; Y Naito; S Sato; A Kitamura; T Okamura; T Sankai; T Shimamoto; M Iida; Y Komachi Journal: Am J Epidemiol Date: 2001-03-01 Impact factor: 4.897
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Authors: J Shepherd; S M Cobbe; I Ford; C G Isles; A R Lorimer; P W MacFarlane; J H McKillop; C J Packard Journal: N Engl J Med Date: 1995-11-16 Impact factor: 91.245