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Literature DB >> 28319057

The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing.

Kyungmin Baeg^1,2, Hiro-Oki Iwakawa^1,2, Yukihide Tomari^1,2.

Abstract

It remains unclear how post-transcriptional gene silencing (PTGS) in plants discriminates aberrant RNAs from canonical messenger RNAs (mRNAs). The key step of plant PTGS is the conversion of aberrant RNAs into double-stranded RNAs by RNA-DEPENDENT RNA POLYMERASE6 (RDR6). Here, we show that RDR6 itself selects aberrant poly(A)-less mRNAs over canonical polyadenylated mRNAs as templates at the initiation step of complementary strand synthesis. This mechanism can be viewed as an innate safeguard against 'self-attack' by PTGS.

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Year: 2017 PMID： 28319057 DOI： 10.1038/nplants.2017.36

Source DB: PubMed Journal: Nat Plants ISSN： 2055-0278 Impact factor: 15.793

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Cited

19 in total

1. Long-Read cDNA Sequencing Enables a "Gene-Like" Transcript Annotation of Transposable Elements.

Authors: Kaushik Panda; R Keith Slotkin
Journal: Plant Cell Date: 2020-07-09 Impact factor: 11.277

Review 2. PhasiRNAs in Plants: Their Biogenesis, Genic Sources, and Roles in Stress Responses, Development, and Reproduction.

Authors: Yuanlong Liu; Chong Teng; Rui Xia; Blake C Meyers
Journal: Plant Cell Date: 2020-08-18 Impact factor: 11.277

3. Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in Arabidopsis.

Authors: Manabu Yoshikawa; Yong-Woon Han; Hirofumi Fujii; Shu Aizawa; Tatsuya Nishino; Masayuki Ishikawa
Journal: Proc Natl Acad Sci U S A Date: 2021-08-24 Impact factor: 11.205

4. An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves.

Authors: Sun Hee Rosenthal; Andrew G Diamos; Hugh S Mason
Journal: Plant Mol Biol Date: 2018-02-10 Impact factor: 4.076

5. Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs.

Authors: Athanasios Dalakouras; Anja Lauter; Alexandra Bassler; Gabi Krczal; Michael Wassenegger
Journal: Planta Date: 2018-09-24 Impact factor: 4.116

The poly(A) tail blocks RDR6 from converting self mRNAs into substrates for gene silencing.

1. Long-Read cDNA Sequencing Enables a "Gene-Like" Transcript Annotation of Transposable Elements.

Review 2. PhasiRNAs in Plants: Their Biogenesis, Genic Sources, and Roles in Stress Responses, Development, and Reproduction.

3. Cooperative recruitment of RDR6 by SGS3 and SDE5 during small interfering RNA amplification in Arabidopsis.

4. An intronless form of the tobacco extensin gene terminator strongly enhances transient gene expression in plant leaves.

5. Transient expression of intron-containing transgenes generates non-spliced aberrant pre-mRNAs that are processed into siRNAs.

Review 6. Biogenesis and regulatory hierarchy of phased small interfering RNAs in plants.

7. High Level Production of Monoclonal Antibodies Using an Optimized Plant Expression System.

8. In vitro RNA-dependent RNA Polymerase Assay Using Arabidopsis RDR6.

9. RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors.

10. Chimeric 3' flanking regions strongly enhance gene expression in plants.