| Literature DB >> 28317760 |
Yawei Ning1, Aihong Yan1, Kun Yang1, Zhixin Wang1, Xingfeng Li2, Yingmin Jia3.
Abstract
Phenyllactic acid (PLA), a phenolic acid phytochemical, is considered to be a promising candidate for use as a chemical preservative due to its broad antimicrobial activity. The antibacterial target of PLA has rarely been reported, thus investigations were performed to elucidate the antibacterial mechanism of PLA against Listeria monocytogenes and Escherichia coli. Flow cytometry analysis stained with propidium iodide (PI) demonstrated that PLA could damage the membrane integrity of L. monocytogenes, while it could not disrupt that of E. coli. The uptake of 1-N-phenylnaphthylamine (NPN) indicated that PLA interrupted the outer membrane permeability of E. coli. Scanning electron microscopy (SEM) observation visualized the damage caused by PLA as morphological changes in L. monocytogenes and E. coli. Fluorescence assays demonstrated that PLA could interact with bacterial genomic DNA in the manner of intercalation. This finding suggested dual antibacterial targets of PLA, namely membrane and genomic DNA.Entities:
Keywords: 1-N-phenylnaphthylamine (PubChem CID: 7013); 3,3′-Dipropylthiadicarbocyanine iodide (PubChem CID: 5706741); Antibacterial activity; DNA; Ethidium bromide (PubChem CID: 14710); Membrane integrity; Phenyllactic acid; Phenyllactic acid (PubChem CID: 3848); Propidium iodide (PubChem CID: 104981); o-Nitrophenyl-β-d-galactopyranoside (PubChem CID: 96647)
Mesh：
Substances：
Year: 2017 PMID： 28317760 DOI： 10.1016/j.foodchem.2017.01.112
Source DB: PubMed Journal: Food Chem ISSN： 0308-8146 Impact factor: 7.514