| Literature DB >> 28316389 |
Sayani Banerjee1, Avijit Patra1, Anjan Mondal1, Harresh Adikesavalu1, Kurva Raghu Ramudu1, Gadadhar Dash1, Siddhartha Narayan Joardar2, Thangapalam Jawahar Abraham1.
Abstract
The present study attempted sequencing the 18S rRNA gene of Myxoboluscatmrigalae infecting the gill lamellae of carp, Cirrhinusmrigala and compared its genetic homology and phylogenetic characteristics with 18S rRNA genes of other Myxobolus spp. The infected fish had up to 3 small, creamy white plasmodia per gill filament with 30-50 spores each. The spore size was 17.90 ± 0.70 × 7.40 ± 0.40 μm. The sporoplasm contained two large nuclei of size 0.57 ± 0.09 μm and no iodinophilous vacuole. The DNA sequence of M.catmrigalae was clustered phylogenetically with other Myxobolus spp. infecting the gills of cyprinids available in GenBank, which showed 77-87 % homogeneity. On the phylogenetic tree, M.catmrigalae (KC933944) was clustered with M.pavlovskii (HM991164) infecting the gill lamellae of silver carp, Hypophthalmichthysmolitrix. The species most closely related to M.catmrigalae in GenBank was M.pavlovskii (AF507973) infecting the gill lamellae of big head carp, Aristichthysnobilis with 87 % homogeneity. This is the first report on molecular characterization of gill lamellae infecting M. catmrigalae.Entities:
Keywords: Aquaculture; Indian major carps; Molecular characterization; Myxoboluscatmrigalae; Myxosporea; Phylogenetic analysis
Year: 2016 PMID： 28316389 PMCID： PMC5339173 DOI： 10.1007/s12639-016-0750-0
Source DB: PubMed Journal: J Parasit Dis ISSN： 0971-7196