Christian Vollmer1, Andreas P M Weber2, Martin Wallenfang1, Till Hoffmann3, Tabea Mettler-Altmann4, Richard Truse1, Inge Bauer1, Olaf Picker1, Alexander M Mathes1,5. 1. Department of Anesthesiology, Düsseldorf University Hospital, Düsseldorf, Germany. 2. Institute of Plant Biochemistry, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany. 3. Department of Hemostaseology, Düsseldorf University Hospital, Düsseldorf, Germany. 4. Plant Metabolism and Metabolomics Laboratory, Cluster of Excellence on Plant Sciences (CEPLAS), Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany. 5. Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, Cologne, Germany.
Abstract
OBJECTIVE: Melatonin improves hepatic perfusion after hemorrhagic shock and may reduce stress-induced gastric lesions. This study was designed to investigate whether pretreatment with melatonin may influence gastric mucosal microcirculatory perfusion (μflow), oxygenation (μHbO2 ), or intestinal barrier function during physiological and hemorrhagic conditions in dogs. METHODS: In a randomized crossover study, five anesthetized foxhounds received melatonin 100 μg kg-1 or vehicle (ethanol 5%) intravenously in the absence or presence of hemorrhagic shock (60 minutes, -20% blood volume). Systemic hemodynamic variables, gastric mucosal perfusion, and oxygenation were recorded continuously; intestinal barrier function was assessed intermittently via xylose absorption. RESULTS: During hemorrhagic shock, melatonin significantly attenuated the decrease in μflow, compared with vehicle (-19±9 vs -43±10 aU, P<.05), without influence on μHbO2 . A significant increase in xylose absorption was detected during hemorrhage in vehicle-treated dogs, compared with sham-operated animals (13±2 vs 8±1 relative amounts, P<.05); this was absent in melatonin-treated animals (6±1 relative amounts). Melatonin did not influence macrocirculation. CONCLUSIONS: Melatonin improves regional blood flow suggesting improved oxygen delivery in gastric mucosa during hemorrhagic shock. This could provide a mechanism for the observed protection of intestinal barrier function in dogs.
OBJECTIVE:Melatonin improves hepatic perfusion after hemorrhagic shock and may reduce stress-induced gastric lesions. This study was designed to investigate whether pretreatment with melatonin may influence gastric mucosal microcirculatory perfusion (μflow), oxygenation (μHbO2 ), or intestinal barrier function during physiological and hemorrhagic conditions in dogs. METHODS: In a randomized crossover study, five anesthetized foxhounds received melatonin 100 μg kg-1 or vehicle (ethanol 5%) intravenously in the absence or presence of hemorrhagic shock (60 minutes, -20% blood volume). Systemic hemodynamic variables, gastric mucosal perfusion, and oxygenation were recorded continuously; intestinal barrier function was assessed intermittently via xylose absorption. RESULTS: During hemorrhagic shock, melatonin significantly attenuated the decrease in μflow, compared with vehicle (-19±9 vs -43±10 aU, P<.05), without influence on μHbO2 . A significant increase in xylose absorption was detected during hemorrhage in vehicle-treated dogs, compared with sham-operated animals (13±2 vs 8±1 relative amounts, P<.05); this was absent in melatonin-treated animals (6±1 relative amounts). Melatonin did not influence macrocirculation. CONCLUSIONS:Melatonin improves regional blood flow suggesting improved oxygen delivery in gastric mucosa during hemorrhagic shock. This could provide a mechanism for the observed protection of intestinal barrier function in dogs.
Authors: Stefan Hof; Carsten Marcus; Anne Kuebart; Jan Schulz; Richard Truse; Annika Raupach; Inge Bauer; Ulrich Flögel; Olaf Picker; Anna Herminghaus; Sebastian Temme Journal: Front Med (Lausanne) Date: 2022-05-16
Authors: Anna Herminghaus; Rebecca Eberhardt; Richard Truse; Jan Schulz; Inge Bauer; Olaf Picker; Christian Vollmer Journal: Front Med (Lausanne) Date: 2018-10-16
Authors: Anoek L I van Leeuwen; Nicole A M Dekker; Elise P Jansma; Christa Boer; Charissa E van den Brom Journal: Microcirculation Date: 2020-08-20 Impact factor: 2.628