| Literature DB >> 2830986 |
C M Preston1, M C Frame, M E Campbell.
Abstract
Transcription of HSV immediate early genes is stimulated by a virus structural protein, Vmw65, in a process that requires specific recognition of the sequence TAATGARAT (R = purine). Upon incubation of nuclear extracts of HSV-infected cells with a short DNA fragment containing TAATGARAT, a novel virus-induced protein-DNA complex (named IEC) was detected. Addition of virion extract, containing Vmw65, to nuclear extract from uninfected cells also resulted in the formation of IEC. Vmw65 is a component of IEC, which contains proteins bound specifically to TAATGARAT. Thus, Vmw65 and cellular factors combine to form a sequence-specific DNA-binding complex. This system provides a model for studies of the regulation of inducible cellular genes.Entities:
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Year: 1988 PMID： 2830986 DOI： 10.1016/s0092-8674(88)80035-7
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582