Literature DB >> 2830889

Characterization of the antigen reactive with anti-Scl-70 antibodies and its application in an enzyme-linked immunosorbent assay.

C Juarez1, J L Vila, C Gelpi, M Agusti, M J Amengual, M A Martinez, J L Rodriguez.   

Abstract

The characteristics of the Scl-70 antigen (topoisomerase I) have been analyzed by means of autoantibodies. This antigen is a DNA-binding protein, dissociable from DNA at 0.3M NaCl and bound to a fraction of DNA that is very sensitive to nucleases. The molecular weight of the antigen is 105,000 daltons, whether dissociation conditions are used or not. Using chicken erythrocytes, and taking advantage of the strong interaction of the antigen with hydroxyapatite, we have designed a simple and fast purification protocol that allows the determination of anti-topoisomerase I antibodies by enzyme-linked immunosorbent assay.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2830889     DOI： 10.1002/art.1780310115

Source DB:  PubMed          Journal:  Arthritis Rheum        ISSN： 0004-3591


  7 in total

1.  Antikinetochore and antitopoisomerase I antibodies in systemic scleroderma: comparative study using immunoblotted recombinant antigens, immunofluorescence, and double immunodiffusion.

Authors:  M Jarzabek-Chorzelska; M Blaszczyk; Z Kolacinska-Strasz; T Chorzelski; S Jabłońska; G G Maul
Journal:  Arch Dermatol Res       Date:  1990       Impact factor: 3.017

2.  Precise quantitation of antinuclear antibodies on HEp-2 cells without the need for serial dilution.

Authors:  P N Hollingsworth; R I Dawkins; J B Peter
Journal:  Clin Diagn Lab Immunol       Date:  1996-07

Review 3.  Antitopoisomerase and anticentromere antibodies in the sclerodermatosus complex.

Authors:  C G Kallenberg
Journal:  Clin Rev Allergy       Date:  1994

4.  Antibodies against a novel nucleolar and cytoplasmic antigen (p105-p42) present in the sera of patients with a subset of rheumatoid arthritis (RA) with signs of scleroderma.

Authors:  M Labrador; A Algueró; C Díaz; C Geli; E Pérez; J García-Valero; J L Rodriguez-Sanchez; C Gelpí
Journal:  Clin Exp Immunol       Date:  1998-11       Impact factor: 4.330

5.  Analysis of human antitopoisomerase-I idiotypes.

Authors:  D Vazquez-Abad; V Pascual; M Zanetti; N F Rothfield
Journal:  J Clin Invest       Date:  1993-09       Impact factor: 14.808

6.  An immunogenetic study of familial scleroderma.

Authors:  M D de Juan; J Belzunegui; I Belmonte; J Barado; M Figueroa; J Cancio; S Vidal; E Cuadrado
Journal:  Ann Rheum Dis       Date:  1994-09       Impact factor: 19.103

Review 7.  Efficiency of a solid-phase chemiluminescence immunoassay for detection of antinuclear and cytoplasmic autoantibodies compared with gold standard immunoprecipitation.

Authors:  Carmen Gelpí; Elena Pérez; Cristina Roldan
Journal:  Auto Immun Highlights       Date:  2014-07-18
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.