Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.

Literature DB >> 2830595

In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.

Abstract

The method for detecting the small fraction (1%) of transcriptionally active SV40 minichromosomes, in the presence of the bulk minichromosomes, (14) has been applied to directly analyze the topology of transcribed and non-transcribed minichromosomal DNA. We show here that DNA of both transcribed and non-transcribed minichromosomes have the same number of supercoils which are constrained by nucleosomes. In addition, minichromosomal DNA of both fractions have a low level of unconstrained supercoils (1-2 extra supercoils) which can be relaxed in vitro by topoisomerase I.

Entities: Species

Mesh：

Substances：

Year: 1988 PMID： 2830595 PMCID： PMC334726 DOI： 10.1093/nar/16.3.895

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors: J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

2. Isolation and partial characterization of a nuclear RNA polymerase - SV40 DNA complex.

Authors: P Gariglio; S Mousset
Journal: FEBS Lett Date: 1975-08-01 Impact factor: 4.124

3. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors: M Shure; D E Pulleyblank; J Vinograd
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

4. Higher order structure of simian virus 40 chromatin.

Authors: U Müller; H Zentgraf; I Eicken; W Keller
Journal: Science Date: 1978-08-04 Impact factor: 47.728

5. Biochemical and ultrastructural analysis of SV40 chromatin.

Authors: W Keller; U Müller; I Eicken; I Wendel; H Zentgraf
Journal: Cold Spring Harb Symp Quant Biol Date: 1978

6. The flexibility and topology of simian virus 40 DNA in minichromosomes.

Authors: C Ambrose; R McLaughlin; M Bina
Journal: Nucleic Acids Res Date: 1987-05-11 Impact factor: 16.971

7. Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells.

Authors: R R Sinden; J O Carlson; D E Pettijohn
Journal: Cell Date: 1980-10 Impact factor: 41.582

8. A nucleosome-free region in SV40 minichromosomes.

Authors: E B Jakobovits; S Bratosin; Y Aloni
Journal: Nature Date: 1980-05-22 Impact factor: 49.962

9. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.

Authors: S Saragosti; G Moyne; M Yaniv
Journal: Cell Date: 1980-05 Impact factor: 41.582

In vitro transcribed SV40 minichromosomes, as the bulk minichromosomes, have a low level of unconstrained negative supercoils.

1. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

2. Isolation and partial characterization of a nuclear RNA polymerase - SV40 DNA complex.

3. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

4. Higher order structure of simian virus 40 chromatin.

5. Biochemical and ultrastructural analysis of SV40 chromatin.

6. The flexibility and topology of simian virus 40 DNA in minichromosomes.

7. Torsional tension in the DNA double helix measured with trimethylpsoralen in living E. coli cells: analogous measurements in insect and human cells.

8. A nucleosome-free region in SV40 minichromosomes.

9. Absence of nucleosomes in a fraction of SV40 chromatin between the origin of replication and the region coding for the late leader RNA.

10. Catenation of DNA by eucaryotic topoisomerase II associated with simian virus 40 minichromosomes.

1. Effects of histone acetylation on chromatin topology in vivo.

2. Enhancer-activated plasmid transcription complexes contain constrained supercoiling.

3. DNA superhelicity enhances the assembly of transcriptionally active chromatin in vitro.

Review 4. DNA-protein interactions and spatial organization of DNA.

5. cis-acting enhancement of RNA polymerase III gene expression in vitro.

6. Thermal unwinding of simian virus 40 transcription complex DNA.

Review 7. Weak interactions in higher-order chromatin organization.