The effect of inhibiting DNA replication in the one-cell mouse embryo.

Inhibition of DNA replication in the 1-cell mouse embryo causes arrest in interphase. In such arrested cells the pattern of protein synthesis progresses as normal through the first cell cycle but does not show the characteristic changes that are associated with mitosis. Synthesis does occur of the 68/70 kd heat shock-like proteins, on the earliest detectable embryonic transcripts synthesised shortly after first cleavage in control embryos. However, the quantitative change in the pattern of protein synthesis, indicative of the major activation of the embryonic genome that normally occurs during G2 of the second cell cycle, is prevented.