Literature DB >> 28305597

Transgenic markers for mammalian chimeras.

James Alexander Thomson1, Davor Solter1.   

Abstract

We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse β-globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA β-globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed.

Entities:  

Keywords:  Chimeras; In situ hybridization; Mice; Transgenic

Year:  1988        PMID： 28305597     DOI： 10.1007/BF00375928

Source DB:  PubMed          Journal:  Rouxs Arch Dev Biol        ISSN： 0930-035X


  7 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  The beneficial effect EDTA on development of mouse one-cell embryos in chemically defined medium.

Authors:  J Abramczuk; D Solter; H Koprowski
Journal:  Dev Biol       Date:  1977-12       Impact factor: 3.582

3.  Dominance of AKR lymphocytes in tetraparental AKR and CBA-T6T6 chimaeras.

Authors:  M Tuffrey; R D Barnes; E P Evans; C E Ford
Journal:  Nat New Biol       Date:  1973-06-13

4.  The biochemistry of differentiation of mouse trophoblast: studies on polyploidy.

Authors:  P W Barlow; M I Sherman
Journal:  J Embryol Exp Morphol       Date:  1972-04

5.  Cell lineage analysis of cerebellar Purkinje cells in mouse chimeras.

Authors:  M L Oster-Granite; J Gearhart
Journal:  Dev Biol       Date:  1981-07-15       Impact factor: 3.582

6.  Identification of embryonic cell lineages in histological sections of M. musculus in-equilibrium M. caroli chimaeras.

Authors:  J Rossant; M Vijh; L D Siracusa; V M Chapman
Journal:  J Embryol Exp Morphol       Date:  1983-02

7.  Localization of low abundance DNA sequences in tissue sections by in situ hybridization.

Authors:  C W Lo
Journal:  J Cell Sci       Date:  1986-03       Impact factor: 5.285
  7 in total
  1 in total

1.  Analysis of cell ploidy in histological sections of mouse tissues by DNA-DNA in situ hybridization with digoxigenin-labelled probes.

Authors:  M Keighren; J D West
Journal:  Histochem J       Date:  1993-01
  1 in total

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