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Literature DB >> 28305355

Fibronectin from the ovary of the sea urchin,Pseudocentrotus depressus.

Abstract

Fibronectin, with a subunit molecular weight of 220,000 daltons, was isolated from the ovary of the sea urchin,Pseudocentrotus depressus, using affinity chromatography on heat-denatured mammalian collagen coupled to Sepharose 4B. The distribution of fibronectin in the sea urchin ovary was examined by indirect immunofluorescence using antifibronectin serum. The basement membrane and the connective tissues exhibited strong fluorescence. The fibronectin was localized closely together with collagen bundles in the sea urchin ovary. Biochemical and immunological examinations indicate that sea urchin fibronectin has similar properties as those of mammalian fibronectin.

Entities: Chemical Disease Gene Species

Keywords: Basement membrane; Collagen; Fibronectin; Sea urchin

Year: 1981 PMID： 28305355 DOI： 10.1007/BF00848399

Source DB: PubMed Journal: Wilehm Roux Arch Dev Biol ISSN： 0340-0794

22 in total

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Authors: J Wartiovaara; S Stenman; A Vaheri
Journal: Differentiation Date: 1976-06-04 Impact factor: 3.880

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Authors: H K Kleinman; E B McGoodwin
Journal: Biochem Biophys Res Commun Date: 1976-09-20 Impact factor: 3.575

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Authors: K M Yamada; K Olden
Journal: Nature Date: 1978-09-21 Impact factor: 49.962

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Authors: S C March; I Parikh; P Cuatrecasas
Journal: Anal Biochem Date: 1974-07 Impact factor: 3.365

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Authors: K Weber; M Osborn
Journal: J Biol Chem Date: 1969-08-25 Impact factor: 5.157

6. Purification of fibronectin from human plasma by affinity chromatography under non-denaturing conditions.

Authors: M Vuento; A Vaheri
Journal: Biochem J Date: 1979-11-01 Impact factor: 3.857

7. Distribution of fibroblast surface antigen in the developing chick embryo.

Authors: E Linder; A Vaheri; E Ruoslahti; J Wartiovaara
Journal: J Exp Med Date: 1975-07-01 Impact factor: 14.307

8. A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells.

Authors: A Vaheri; E Ruoslahti; B Westermark; J Ponten
Journal: J Exp Med Date: 1976-01-01 Impact factor: 14.307

9. Distribution of a major connective tissue protein, fibronectin, in normal human tissues.

Authors: S Stenman; A Vaheri
Journal: J Exp Med Date: 1978-04-01 Impact factor: 14.307

10. Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.

Authors: E Anderson
Journal: J Cell Biol Date: 1968-05 Impact factor: 10.539

5 in total

5. Immunocytochemical study of fibronectin in the sea lamprey, Petromyzon marinus, and the Atlantic hagfish, Myxine glutinosa.

Authors: G M Wright
Journal: Cell Tissue Res Date: 1986 Impact factor: 5.249