Literature DB >> 28305159

A new method for the isolation of plasma membranes from amphibian embryos.

Horst Grunz1.   

Abstract

A method for the isolation of plasma membranes is described. N-Succinimidyl 3-(2-pyridyldithio) propionate (SPDP), a heterobifunctional reagent, is covalently linked to protein amino groups in plasma membranes of intact cells. After homogenization of the cells the plasma membranes can be separated from other cell components by selective coupling to reduced Thiopropyl-Sepharose 6B and then recovered after treatment with 2-mercaptoethanol.

Entities:  

Keywords:  Amphibians; Early embryonic development; Plasma membranes

Year:  1980        PMID： 28305159     DOI： 10.1007/BF00848614

Source DB:  PubMed          Journal:  Wilehm Roux Arch Dev Biol        ISSN： 0340-0794


  5 in total

1.  The differentiation of isolated amphibian ectoderm with or without treatment with an inductor : A scanning electron microscop study.

Authors:  Horst Grunz; Anne-Marie Multier-Lajous; Rolf Herbst; Gisela Arkenberg
Journal:  Wilehm Roux Arch Dev Biol       Date:  1975-12

2.  Changes of the cell surface charge of amphibian ectoderm after induction.

Authors:  Horst Grunz; Juliane Staubach
Journal:  Wilehm Roux Arch Dev Biol       Date:  1979-03

3.  Aggregation of embryonic cells in a serum-free medium and its inhibition at suboptimal temperatures.

Authors:  A A Moscona; M H Moscona
Journal:  Exp Cell Res       Date:  1966-03       Impact factor: 3.905

4.  [Inhibition of aggregation of dissociated embryonic Amphibian cells by inhibitors of protein and RNA synthesis].

Authors:  Horst Grunz
Journal:  Wilhelm Roux Arch Entwickl Mech Org       Date:  1969-06

5.  A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.

Authors:  J H VENABLE; R COGGESHALL
Journal:  J Cell Biol       Date:  1965-05       Impact factor: 10.539
  5 in total
  1 in total

1.  Isolation of plasma membranes from Xenopus embryos.

Authors:  Günther Bretzel; Jutta Janeczek; Jochen Born; Manorama John; Heinz Tiedemann; Hildegard Tiedemann
Journal:  Rouxs Arch Dev Biol       Date:  1986-02
  1 in total

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