Vitamin D sites of action in the pituitary studied by combined autoradiography-immunohistochemistry.

Adult male and female mice under normal diet were injected with 3H 1,25(OH)2 vitamin D3 and sacrificed 3.5 h afterwards. Autoradiograms were prepared according to our thaw-mount technique and stained with antibodies to pituitary hormones. Thyrotropes showed strong and extensive nuclear concentration of radioactivity: about 90% of the immunostained thyrotropes were labeled. Lactotropes, somatotropes and gonadotropes showed no or only weak nuclear radioactivity: a subpopulation of 5%-10% of each of these immunostained cell types displayed nuclear labeling that was weak when compared to thyrotropes. Neural lobe pituicytes also showed weak to intermediate nuclear labeling. The results indicate a presence of nuclear receptors for 1,25(OH)2 vitamin D3 in pituitary cell types and suggest direct but differential genomic effects of 1,25(OH)2 vitamin D3 on pituitary hormone secretion. Evidence further suggests the existence of a vitamin D regulated brain-pituitary-thyroid axis.